Hyperbaric oxygen reduces basal lamina degradation after transient focal cerebral ischemia in rats

Roland Veltkamp*, Katja Bieber, Simone Wagner, Christopher Beynon, Dirk A. Siebing, Claudia Veltkamp, Markus Schwaninger, Hugo H. Marti

*Corresponding author for this work
35 Citations (Scopus)


Hyperbaric oxygen (HBO) has been shown to preserve the integrity of the blood-brain barrier after cerebral ischemia. However, the underlying molecular mechanisms are currently unknown. We examined the effect of HBO on postischemic expression of the basal laminar component laminin-5 and on plasma matrix metalloproteinase-9 (MMP) levels. Wistar rats underwent occlusion of the middle cerebral artery (MCAO) for 2 h. With a delay of 45 min after filament introduction, animals breathed either 100% O2 at 1.0 atmosphere absolute (ata; NBO) or at 3.0 ata (HBO) for 1 h in an HBO chamber. Laminin-5 expression was quantified on immunohistochemical sections after 24 h of reperfusion. Plasma MMP-9 levels were measured using gelatin zymography before MCAO as well as 0, 6 and 24 h after reperfusion. Immunohistochemistry 24 h after ischemia revealed a decrease of vascular laminin-5 staining in the ischemic striatum to 43 ± 26% of the contralateral hemisphere in the NBO group which was significantly attenuated to 73 ± 31% in the HBO group. Densitometric analysis of zymography bands yielded significantly larger plasma MMP-9 levels in the NBO group compared to the HBO group 24 h after ischemia. In conclusion, HBO therapy attenuates ischemic degradation of cerebral microvascular laminin-5 and blocks postischemic plasma MMP-9 upregulation.

Original languageEnglish
JournalBrain Research
Issue number1
Pages (from-to)231-237
Number of pages7
Publication statusPublished - 03.03.2006

Research Areas and Centers

  • Academic Focus: Center for Brain, Behavior and Metabolism (CBBM)


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