Human macrophages simultaneously express membrane-C1q and Fc-receptors for IgG

Patrick Terheyden; Michael Loos; Stephan Störkel; Marcus Kaul

doi:10.1016/j.imlet.2005.06.002

Human macrophages simultaneously express membrane-C1q and Fc-receptors for IgG

Patrick Terheyden, Michael Loos, Stephan Störkel, Marcus Kaul^*

^*Corresponding author for this work

Department of Dermatology, Allergology and Venereology

4 Citations (Scopus)

Abstract

Membrane C1q (mC1q) of macrophages (MΦ) is a precursor of the IgG-binding serum protein C1q. Thus, mC1q potentially provides one of several Fcγ binding sites of mature MΦ and we analyzed whether simultaneous expression occurs of established receptors for IgG, FcγRI, II, and III, and mC1q during in vitro differentiation of MΦ. Using flow cytometry, immunoprecipitation combined with Western blotting and Northern blot analysis mC1q was hardly detected in freshly isolated blood monocytes, but increasingly in developing monocyte-derived MΦ. Laser scanning fluorescence microscopy confirmed the membrane localization of mC1q. Two-color-staining flow cytometry experiments indicated that mC1q and all three types of FcγRs are simultaneously expressed on mature monocyte-derived MΦ. A high correlation was found for the expression of mC1q and FcγRs, in particular FcγRII, but not mC1q and CD14, another marker of monocytes/MΦ.

Original language	English
Journal	Immunology Letters
Volume	101
Issue number	2
Pages (from-to)	202-209
Number of pages	8
ISSN	0165-2478
DOIs	https://doi.org/10.1016/j.imlet.2005.06.002
Publication status	Published - 15.11.2005

Funding

We thank Dr. Trudy Wassenaar for expert help in performing Northern Blot analyses, and Sabine Pauls and Stefanie Marx for their excellent technical assistance. This work was supported by the Deutsche Forschungsgemeinschaft: SFB 311, D1, and the NIH (R01 NS050621 to M.K.).

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SDG 3 Good Health and Well-being

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10.1016/j.imlet.2005.06.002

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title = "Human macrophages simultaneously express membrane-C1q and Fc-receptors for IgG",

abstract = "Membrane C1q (mC1q) of macrophages (MΦ) is a precursor of the IgG-binding serum protein C1q. Thus, mC1q potentially provides one of several Fcγ binding sites of mature MΦ and we analyzed whether simultaneous expression occurs of established receptors for IgG, FcγRI, II, and III, and mC1q during in vitro differentiation of MΦ. Using flow cytometry, immunoprecipitation combined with Western blotting and Northern blot analysis mC1q was hardly detected in freshly isolated blood monocytes, but increasingly in developing monocyte-derived MΦ. Laser scanning fluorescence microscopy confirmed the membrane localization of mC1q. Two-color-staining flow cytometry experiments indicated that mC1q and all three types of FcγRs are simultaneously expressed on mature monocyte-derived MΦ. A high correlation was found for the expression of mC1q and FcγRs, in particular FcγRII, but not mC1q and CD14, another marker of monocytes/MΦ.",

author = "Patrick Terheyden and Michael Loos and Stephan St{\"o}rkel and Marcus Kaul",

note = "Funding Information: We thank Dr. Trudy Wassenaar for expert help in performing Northern Blot analyses, and Sabine Pauls and Stefanie Marx for their excellent technical assistance. This work was supported by the Deutsche Forschungsgemeinschaft: SFB 311, D1, and the NIH (R01 NS050621 to M.K.).",

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doi = "10.1016/j.imlet.2005.06.002",

language = "English",

volume = "101",

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journal = "Immunology Letters",

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T1 - Human macrophages simultaneously express membrane-C1q and Fc-receptors for IgG

AU - Terheyden, Patrick

AU - Loos, Michael

AU - Störkel, Stephan

AU - Kaul, Marcus

N1 - Funding Information: We thank Dr. Trudy Wassenaar for expert help in performing Northern Blot analyses, and Sabine Pauls and Stefanie Marx for their excellent technical assistance. This work was supported by the Deutsche Forschungsgemeinschaft: SFB 311, D1, and the NIH (R01 NS050621 to M.K.).

PY - 2005/11/15

Y1 - 2005/11/15

N2 - Membrane C1q (mC1q) of macrophages (MΦ) is a precursor of the IgG-binding serum protein C1q. Thus, mC1q potentially provides one of several Fcγ binding sites of mature MΦ and we analyzed whether simultaneous expression occurs of established receptors for IgG, FcγRI, II, and III, and mC1q during in vitro differentiation of MΦ. Using flow cytometry, immunoprecipitation combined with Western blotting and Northern blot analysis mC1q was hardly detected in freshly isolated blood monocytes, but increasingly in developing monocyte-derived MΦ. Laser scanning fluorescence microscopy confirmed the membrane localization of mC1q. Two-color-staining flow cytometry experiments indicated that mC1q and all three types of FcγRs are simultaneously expressed on mature monocyte-derived MΦ. A high correlation was found for the expression of mC1q and FcγRs, in particular FcγRII, but not mC1q and CD14, another marker of monocytes/MΦ.

AB - Membrane C1q (mC1q) of macrophages (MΦ) is a precursor of the IgG-binding serum protein C1q. Thus, mC1q potentially provides one of several Fcγ binding sites of mature MΦ and we analyzed whether simultaneous expression occurs of established receptors for IgG, FcγRI, II, and III, and mC1q during in vitro differentiation of MΦ. Using flow cytometry, immunoprecipitation combined with Western blotting and Northern blot analysis mC1q was hardly detected in freshly isolated blood monocytes, but increasingly in developing monocyte-derived MΦ. Laser scanning fluorescence microscopy confirmed the membrane localization of mC1q. Two-color-staining flow cytometry experiments indicated that mC1q and all three types of FcγRs are simultaneously expressed on mature monocyte-derived MΦ. A high correlation was found for the expression of mC1q and FcγRs, in particular FcγRII, but not mC1q and CD14, another marker of monocytes/MΦ.

UR - https://www.scopus.com/pages/publications/25444487479

U2 - 10.1016/j.imlet.2005.06.002

DO - 10.1016/j.imlet.2005.06.002

M3 - Journal articles

C2 - 16023736

AN - SCOPUS:25444487479

SN - 0165-2478

VL - 101

SP - 202

EP - 209

JO - Immunology Letters

JF - Immunology Letters

IS - 2

ER -

Human macrophages simultaneously express membrane-C1q and Fc-receptors for IgG

Abstract

Funding

UN SDGs

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