TY - JOUR
T1 - Human macrophages simultaneously express membrane-C1q and Fc-receptors for IgG
AU - Terheyden, Patrick
AU - Loos, Michael
AU - Störkel, Stephan
AU - Kaul, Marcus
N1 - Funding Information:
We thank Dr. Trudy Wassenaar for expert help in performing Northern Blot analyses, and Sabine Pauls and Stefanie Marx for their excellent technical assistance. This work was supported by the Deutsche Forschungsgemeinschaft: SFB 311, D1, and the NIH (R01 NS050621 to M.K.).
PY - 2005/11/15
Y1 - 2005/11/15
N2 - Membrane C1q (mC1q) of macrophages (MΦ) is a precursor of the IgG-binding serum protein C1q. Thus, mC1q potentially provides one of several Fcγ binding sites of mature MΦ and we analyzed whether simultaneous expression occurs of established receptors for IgG, FcγRI, II, and III, and mC1q during in vitro differentiation of MΦ. Using flow cytometry, immunoprecipitation combined with Western blotting and Northern blot analysis mC1q was hardly detected in freshly isolated blood monocytes, but increasingly in developing monocyte-derived MΦ. Laser scanning fluorescence microscopy confirmed the membrane localization of mC1q. Two-color-staining flow cytometry experiments indicated that mC1q and all three types of FcγRs are simultaneously expressed on mature monocyte-derived MΦ. A high correlation was found for the expression of mC1q and FcγRs, in particular FcγRII, but not mC1q and CD14, another marker of monocytes/MΦ.
AB - Membrane C1q (mC1q) of macrophages (MΦ) is a precursor of the IgG-binding serum protein C1q. Thus, mC1q potentially provides one of several Fcγ binding sites of mature MΦ and we analyzed whether simultaneous expression occurs of established receptors for IgG, FcγRI, II, and III, and mC1q during in vitro differentiation of MΦ. Using flow cytometry, immunoprecipitation combined with Western blotting and Northern blot analysis mC1q was hardly detected in freshly isolated blood monocytes, but increasingly in developing monocyte-derived MΦ. Laser scanning fluorescence microscopy confirmed the membrane localization of mC1q. Two-color-staining flow cytometry experiments indicated that mC1q and all three types of FcγRs are simultaneously expressed on mature monocyte-derived MΦ. A high correlation was found for the expression of mC1q and FcγRs, in particular FcγRII, but not mC1q and CD14, another marker of monocytes/MΦ.
UR - http://www.scopus.com/inward/record.url?scp=25444487479&partnerID=8YFLogxK
U2 - 10.1016/j.imlet.2005.06.002
DO - 10.1016/j.imlet.2005.06.002
M3 - Journal articles
C2 - 16023736
AN - SCOPUS:25444487479
SN - 0165-2478
VL - 101
SP - 202
EP - 209
JO - Immunology Letters
JF - Immunology Letters
IS - 2
ER -
