Hochauflösende 2D-Gelelektrophorese von Knochengewebsproben

Timo Gemoll; Tilman Laubert; Cornelius Grimme; Uwe J. Roblick; Jens K. Habermann

doi:10.1007/s12268-012-0224-6

Hochauflösende 2D-Gelelektrophorese von Knochengewebsproben

Timo Gemoll, Tilman Laubert, Cornelius Grimme, Uwe J. Roblick, Jens K. Habermann

Clinic of Surgery

Abstract

Two-dimensional gel electrophoresis (2-DE) is commonly used to analyze and separate complex mixtures of intact proteins. It delivers a map of proteins which reflects changes in protein expression level, isoforms and post-translational modifications. In this protocol, we describe an optimized workflow with standardized sample preparation for the 2-DE to exploit all methodical and technical potentials.

Original language	German
Journal	BioSpektrum
Volume	18
Issue number	5
Pages (from-to)	520-521
Number of pages	2
ISSN	0947-0867
DOIs	https://doi.org/10.1007/s12268-012-0224-6
Publication status	Published - 09.2012

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This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/s12268-012-0224-6

http://www.mendeley.com/research/hochaufl%C3%B6sende-2dgelelektrophorese-von-knochengewebsproben

Cite this

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title = "Hochaufl{\"o}sende 2D-Gelelektrophorese von Knochengewebsproben",

abstract = "Two-dimensional gel electrophoresis (2-DE) is commonly used to analyze and separate complex mixtures of intact proteins. It delivers a map of proteins which reflects changes in protein expression level, isoforms and post-translational modifications. In this protocol, we describe an optimized workflow with standardized sample preparation for the 2-DE to exploit all methodical and technical potentials.",

author = "Timo Gemoll and Tilman Laubert and Cornelius Grimme and Roblick, {Uwe J.} and Habermann, {Jens K.}",

year = "2012",

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AU - Gemoll, Timo

AU - Laubert, Tilman

AU - Grimme, Cornelius

AU - Roblick, Uwe J.

AU - Habermann, Jens K.

PY - 2012/9

Y1 - 2012/9

N2 - Two-dimensional gel electrophoresis (2-DE) is commonly used to analyze and separate complex mixtures of intact proteins. It delivers a map of proteins which reflects changes in protein expression level, isoforms and post-translational modifications. In this protocol, we describe an optimized workflow with standardized sample preparation for the 2-DE to exploit all methodical and technical potentials.

AB - Two-dimensional gel electrophoresis (2-DE) is commonly used to analyze and separate complex mixtures of intact proteins. It delivers a map of proteins which reflects changes in protein expression level, isoforms and post-translational modifications. In this protocol, we describe an optimized workflow with standardized sample preparation for the 2-DE to exploit all methodical and technical potentials.

U2 - 10.1007/s12268-012-0224-6

DO - 10.1007/s12268-012-0224-6

M3 - Zeitschriftenaufsätze

SN - 0947-0867

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SP - 520

EP - 521

JO - BioSpektrum

JF - BioSpektrum

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