Hochauflösende 2D-Gelelektrophorese von Knochengewebsproben

Abstract

Two-dimensional gel electrophoresis (2-DE) is commonly used to analyze and separate complex mixtures of intact proteins. It delivers a map of proteins which reflects changes in protein expression level, isoforms and post-translational modifications. In this protocol, we describe an optimized workflow with standardized sample preparation for the 2-DE to exploit all methodical and technical potentials.
Original languageGerman
JournalBioSpektrum
Volume18
Issue number5
Pages (from-to)520-521
Number of pages2
ISSN0947-0867
DOIs
Publication statusPublished - 09.2012

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