High-resolution rectoscopy using MHz optical coherence tomography: a step towards real time 3D endoscopy

Abstract

Colonoscopy and endoscopic ultrasound play pivotal roles in the assessment of rectal diseases, especially rectal cancer and inflammatory bowel diseases. Optical coherence tomography (OCT) offers a superior depth resolution, which is a critical factor for individualizing the therapeutic concept and evaluating the therapy response. We developed two distinct rectoscope prototypes, which were integrated into a 1300 nm MHz-OCT system constructed at our facility. The rapid rotation of the distal scanning probe at 40,000 revolutions per minute facilitates a 667 Hz OCT frame rate, enabling real-time endoscopic imaging of large areas. The performance of these OCT-rectoscopes was assessed in an ex vivo porcine colon and a post mortem human in-situ colon. The OCT-rectoscope consistently distinguished various layers of the intestinal wall, identified gut-associated lymphatic tissue, and visualized a rectal polyp during the imaging procedure with 3D-reconstruction in real time. Subsequent histological examination confirmed these findings. The body donor was preserved using an ethanol-glycerol-lysoformin-based technique for true-to-life tissue consistency. We could demonstrate that the novel MHZ-OCT-rectoscope effectively discriminates rectal wall layers and crucial tissue characteristics in a post mortem human colon in-situ. This real-time-3D-OCT holds promise as a valuable future diagnostic tool for assessing disease state and therapy response on-site in rectal diseases.

Similar content being viewed by otherscrucial tissue characteristics in a post mortem human colon in‑situ. This real‑time‑3D‑OCT holds
promise as a valuable future diagnostic tool for assessing disease state and therapy response on‑site in rectal diseases.
Original languageEnglish
Article number4672
JournalScientific Reports
Volume14
Issue number1
Pages (from-to)4672
ISSN2045-2322
DOIs
Publication statusPublished - 26.02.2024

Research Areas and Centers

  • Academic Focus: Biomedical Engineering

DFG Research Classification Scheme

  • 205-32 Medical Physics, Biomedical Engineering
  • 205-33 Anatomy

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