TY - JOUR
T1 - High interleukin-13 production by phytohaemagglutinin- and Der p 1-stimulated cord blood mononuclear cells is associated with the subsequent development of atopic dermatitis at the age of 3 years
AU - Lange, J.
AU - Ngoumou, G.
AU - Berkenheide, S.
AU - Moseler, M.
AU - Mattes, J.
AU - Kuehr, J.
AU - Kopp, M. V.
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2003/11
Y1 - 2003/11
N2 - Objective: The aim of our study was to conduct a prospective investigation into the potential association of cord blood proliferative response and cytokine production in response to various stimuli on the development of atopic dermatitis (AD) at the age of 3 years. Methods: Cord blood mononuclear cells (CBMC) from 40 healthy term neonates were isolated. The proliferative response of CBMC stimulated with IL-2, betalactoglobulin (BLG) and house dust mite allergen (Der p 1) was assessed by liquid scintillation counting and the stimulation index (SI) was calculated. The cytokines interleukin (IL-)13, interferon (IFN-)γ, IL-10 and IL-18 in the cell culture supernatants in response to phytohaemagglutinin (PHA), Der p 1 and BLG were measured using the ELISA technique. After 3 years, symptoms of AD were obtained with a questionnaire completed by the parents. Results: We observed significantly higher IL-13 levels in response to PHA in children who subsequently developed symptoms of AD (S: median, 291 pg/mL) compared with asymptomatic children (No-S: 149 pg/mL; P = 0.021, Wilcoxon test). Similarly, in response to Der p 1 significantly higher IL-13 levels were observed in symptomatic children (S: 168.6; No-S: 61.6 pg/mL; P = 0.0084). In response to BLG, IL-13 levels were 287.2 (S) and 123.6 pg/mL (No-S; P = 0.19). No significant differences were found when comparing the IFN-γ levels in CBMC cultures stimulated with PHA (S: 10.2; No-S: 17.6 IU/L; P = 0.78), Der p 1 (S: 307.6; No-S: 616.2 IU/L; P = 0.2) or BLG (S: 18; No-S: 28.5 IU/L; P = 0.83; Fig. 2). The IL-18 and IL-10 levels and the stimulation index in response to IL-2, BLG and Der p 1 showed no significant difference between children who subsequently developed symptoms of AD and asymptomatic children. Conclusion: Our data suggest that enhanced IL-13 levels at birth are associated with the subsequent development of atopic symptoms at the age of 3 years.
AB - Objective: The aim of our study was to conduct a prospective investigation into the potential association of cord blood proliferative response and cytokine production in response to various stimuli on the development of atopic dermatitis (AD) at the age of 3 years. Methods: Cord blood mononuclear cells (CBMC) from 40 healthy term neonates were isolated. The proliferative response of CBMC stimulated with IL-2, betalactoglobulin (BLG) and house dust mite allergen (Der p 1) was assessed by liquid scintillation counting and the stimulation index (SI) was calculated. The cytokines interleukin (IL-)13, interferon (IFN-)γ, IL-10 and IL-18 in the cell culture supernatants in response to phytohaemagglutinin (PHA), Der p 1 and BLG were measured using the ELISA technique. After 3 years, symptoms of AD were obtained with a questionnaire completed by the parents. Results: We observed significantly higher IL-13 levels in response to PHA in children who subsequently developed symptoms of AD (S: median, 291 pg/mL) compared with asymptomatic children (No-S: 149 pg/mL; P = 0.021, Wilcoxon test). Similarly, in response to Der p 1 significantly higher IL-13 levels were observed in symptomatic children (S: 168.6; No-S: 61.6 pg/mL; P = 0.0084). In response to BLG, IL-13 levels were 287.2 (S) and 123.6 pg/mL (No-S; P = 0.19). No significant differences were found when comparing the IFN-γ levels in CBMC cultures stimulated with PHA (S: 10.2; No-S: 17.6 IU/L; P = 0.78), Der p 1 (S: 307.6; No-S: 616.2 IU/L; P = 0.2) or BLG (S: 18; No-S: 28.5 IU/L; P = 0.83; Fig. 2). The IL-18 and IL-10 levels and the stimulation index in response to IL-2, BLG and Der p 1 showed no significant difference between children who subsequently developed symptoms of AD and asymptomatic children. Conclusion: Our data suggest that enhanced IL-13 levels at birth are associated with the subsequent development of atopic symptoms at the age of 3 years.
UR - http://www.scopus.com/inward/record.url?scp=0242552784&partnerID=8YFLogxK
U2 - 10.1046/j.1365-2222.2003.01789.x
DO - 10.1046/j.1365-2222.2003.01789.x
M3 - Journal articles
C2 - 14616866
AN - SCOPUS:0242552784
SN - 0954-7894
VL - 33
SP - 1537
EP - 1543
JO - Clinical and Experimental Allergy
JF - Clinical and Experimental Allergy
IS - 11
ER -
