TY - JOUR
T1 - HDAC2 and TXNL1 distinguish aneuploid from diploid colorectal cancers
AU - Gemoll, Timo
AU - Roblick, Uwe J.
AU - Szymczak, Silke
AU - Braunschweig, Till
AU - Becker, Susanne
AU - Igl, Bernd Wolfgang
AU - Bruch, Hans Peter
AU - Ziegler, Andreas
AU - Hellman, Ulf
AU - Difilippantonio, Michael J.
AU - Ried, Thomas
AU - Jörnvall, Hans
AU - Auer, Gert
AU - Habermann, Jens K.
PY - 2011/10/1
Y1 - 2011/10/1
N2 - DNA aneuploidy has been identified as a prognostic factor for epithelial malignancies. Further understanding of the translation of DNA aneuploidy into protein expression will help to define novel biomarkers to improve therapies and prognosis. DNA ploidy was assessed by image cytometry. Comparison of gel-electrophoresisbased protein expression patterns of three diploid and four aneuploid colorectal cancer cell lines detected 64 ploidyassociated proteins. Proteins were identified by mass spectrometry and subjected to Ingenuity Pathway Analysis resulting in two overlapping high-ranked networks maintaining Cellular Assembly and Organization, Cell Cycle, and Cellular Growth and Proliferation. CAPZA1, TXNL1, and HDAC2 were significantly validated by Western blotting in cell lines and the latter two showed expression differences also in clinical samples using a tissue microarray of normal mucosa (n = 19), diploid (n = 31), and aneuploid (n = 47) carcinomas. The results suggest that distinct protein expression patterns, affecting TXNL1 and HDAC2, distinguish aneuploid with poor prognosis from diploid colorectal cancers.
AB - DNA aneuploidy has been identified as a prognostic factor for epithelial malignancies. Further understanding of the translation of DNA aneuploidy into protein expression will help to define novel biomarkers to improve therapies and prognosis. DNA ploidy was assessed by image cytometry. Comparison of gel-electrophoresisbased protein expression patterns of three diploid and four aneuploid colorectal cancer cell lines detected 64 ploidyassociated proteins. Proteins were identified by mass spectrometry and subjected to Ingenuity Pathway Analysis resulting in two overlapping high-ranked networks maintaining Cellular Assembly and Organization, Cell Cycle, and Cellular Growth and Proliferation. CAPZA1, TXNL1, and HDAC2 were significantly validated by Western blotting in cell lines and the latter two showed expression differences also in clinical samples using a tissue microarray of normal mucosa (n = 19), diploid (n = 31), and aneuploid (n = 47) carcinomas. The results suggest that distinct protein expression patterns, affecting TXNL1 and HDAC2, distinguish aneuploid with poor prognosis from diploid colorectal cancers.
UR - http://www.scopus.com/inward/record.url?scp=80052918984&partnerID=8YFLogxK
U2 - 10.1007/s00018-011-0628-3
DO - 10.1007/s00018-011-0628-3
M3 - Journal articles
C2 - 21290163
AN - SCOPUS:80052918984
SN - 1420-682X
VL - 68
SP - 3261
EP - 3274
JO - Cellular and Molecular Life Sciences
JF - Cellular and Molecular Life Sciences
IS - 19
ER -
