Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

Andrea Cossarizza; Hyun Dong Chang; Andreas Radbruch; Sergio Abrignani; Richard Addo; Mübeccel Akdis; Immanuel Andrä; Francesco Andreata; Francesco Annunziato; Eduardo Arranz; Petra Bacher; Sudipto Bari; Vincenzo Barnaba; Joana Barros-Martins; Dirk Baumjohann; Cristian G. Beccaria; David Bernardo; Dominic A. Boardman; Jessica Borger; Chotima Böttcher; Leonie Brockmann; Marie Burns; Dirk H. Busch; Garth Cameron; Ilenia Cammarata; Antonino Cassotta; Yinshui Chang; Fernando Gabriel Chirdo; Eleni Christakou; Luka Čičin-Šain; Laura Cook; Alexandra J. Corbett; Rebecca Cornelis; Lorenzo Cosmi; Martin S. Davey; Sara De Biasi; Gabriele De Simone; Genny del Zotto; Michael Delacher; Francesca Di Rosa; James Di Santo; Andreas Diefenbach; Jun Dong; Thomas Dörner; Regine J. Dress; Charles Antoine Dutertre; Sidonia B.G. Eckle; Pascale Eede; Maximilien Evrard; Christine S. Falk; Markus Feuerer; Simon Fillatreau; Aida Fiz-Lopez; Marie Follo; Gemma A. Foulds; Julia Fröbel; Nicola Gagliani; Giovanni Galletti; Anastasia Gangaev; Natalio Garbi; José Antonio Garrote; Jens Geginat; Nicholas A. Gherardin; Lara Gibellini; Florent Ginhoux; Dale I. Godfrey; Paola Gruarin; Claudia Haftmann; Leo Hansmann; Christopher M. Harpur; Adrian C. Hayday; Guido Heine; Daniela Carolina Hernández; Martin Herrmann; Oliver Hoelsken; Qing Huang; Samuel Huber; Johanna E. Huber; Jochen Huehn; Michael Hundemer; William Y.K. Hwang; Matteo Iannacone; Sabine M. Ivison; Hans Martin Jäck; Peter K. Jani; Baerbel Keller; Nina Kessler; Steven Ketelaars; Laura Knop; Jasmin Knopf; Hui Fern Koay; Katja Kobow; Katharina Kriegsmann; H. Kristyanto; Andreas Krueger; Jenny F. Kuehne; Heike Kunze-Schumacher; Pia Kvistborg; Immanuel Kwok; Daniela Latorre; Daniel Lenz; Megan K. Levings; Andreia C. Lino; Francesco Liotta; Heather M. Long; Enrico Lugli; Katherine N. MacDonald; Laura Maggi; Mala K. Maini; Florian Mair; Calin Manta; Rudolf Armin Manz; Mir Farzin Mashreghi; Alessio Mazzoni; James McCluskey; Henrik E. Mei; Fritz Melchers; Susanne Melzer; Dirk Mielenz; Leticia Monin; Lorenzo Moretta; Gabriele Multhoff; Luis Enrique Muñoz; Miguel Muñoz-Ruiz; Franziska Muscate; Ambra Natalini; Katrin Neumann; Lai Guan Ng; Antonia Niedobitek; Jana Niemz; Larissa Nogueira Almeida; Samuele Notarbartolo; Lennard Ostendorf; Laura J. Pallett; Amit A. Patel; Gulce Itir Percin; Giovanna Peruzzi; Marcello Pinti; A. Graham Pockley; Katharina Pracht; Immo Prinz; Irma Pujol-Autonell; Nadia Pulvirenti; Linda Quatrini; Kylie M. Quinn; Helena Radbruch; Hefin Rhys; Maria B. Rodrigo; Chiara Romagnani; Carina Saggau; Shimon Sakaguchi; Federica Sallusto; Lieke Sanderink; Inga Sandrock; Christine Schauer; Alexander Scheffold; Hans U. Scherer; Matthias Schiemann; Frank A. Schildberg; Kilian Schober; Janina Schoen; Wolfgang Schuh; Thomas Schüler; Axel R. Schulz; Sebastian Schulz; Julia Schulze; Sonia Simonetti; Jeeshan Singh; Katarzyna M. Sitnik; Regina Stark; Sarah Starossom; Christina Stehle; Franziska Szelinski; Leonard Tan; Attila Tarnok; Julia Tornack; Timothy I.M. Tree; Jasper J.P. van Beek; Willem van de Veen; Klaas van Gisbergen; Chiara Vasco; Nikita A. Verheyden; Anouk von Borstel; Kirsten A. Ward-Hartstonge; Klaus Warnatz; Claudia Waskow; Annika Wiedemann; Anneke Wilharm; James Wing; Oliver Wirz; Jens Wittner; Jennie H.M. Yang; Juhao Yang

doi:10.1002/eji.202170126

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

Andrea Cossarizza^*, Hyun Dong Chang^*, Andreas Radbruch, Sergio Abrignani, Richard Addo, Mübeccel Akdis, Immanuel Andrä, Francesco Andreata, Francesco Annunziato, Eduardo Arranz, Petra Bacher, Sudipto Bari, Vincenzo Barnaba, Joana Barros-Martins, Dirk Baumjohann, Cristian G. Beccaria, David Bernardo, Dominic A. Boardman, Jessica Borger, Chotima BöttcherLeonie Brockmann, Marie Burns, Dirk H. Busch, Garth Cameron, Ilenia Cammarata, Antonino Cassotta, Yinshui Chang, Fernando Gabriel Chirdo, Eleni Christakou, Luka Čičin-Šain, Laura Cook, Alexandra J. Corbett, Rebecca Cornelis, Lorenzo Cosmi, Martin S. Davey, Sara De Biasi, Gabriele De Simone, Genny del Zotto, Michael Delacher, Francesca Di Rosa, James Di Santo, Andreas Diefenbach, Jun Dong, Thomas Dörner, Regine J. Dress, Charles Antoine Dutertre, Sidonia B.G. Eckle, Pascale Eede, Maximilien Evrard, Christine S. Falk, Markus Feuerer, Simon Fillatreau, Aida Fiz-Lopez, Marie Follo, Gemma A. Foulds, Julia Fröbel, Nicola Gagliani, Giovanni Galletti, Anastasia Gangaev, Natalio Garbi, José Antonio Garrote, Jens Geginat, Nicholas A. Gherardin, Lara Gibellini, Florent Ginhoux, Dale I. Godfrey, Paola Gruarin, Claudia Haftmann, Leo Hansmann, Christopher M. Harpur, Adrian C. Hayday, Guido Heine, Daniela Carolina Hernández, Martin Herrmann, Oliver Hoelsken, Qing Huang, Samuel Huber, Johanna E. Huber, Jochen Huehn, Michael Hundemer, William Y.K. Hwang, Matteo Iannacone, Sabine M. Ivison, Hans Martin Jäck, Peter K. Jani, Baerbel Keller, Nina Kessler, Steven Ketelaars, Laura Knop, Jasmin Knopf, Hui Fern Koay, Katja Kobow, Katharina Kriegsmann, H. Kristyanto, Andreas Krueger, Jenny F. Kuehne, Heike Kunze-Schumacher, Pia Kvistborg, Immanuel Kwok, Daniela Latorre, Daniel Lenz, Megan K. Levings, Andreia C. Lino, Francesco Liotta, Heather M. Long, Enrico Lugli, Katherine N. MacDonald, Laura Maggi, Mala K. Maini, Florian Mair, Calin Manta, Rudolf Armin Manz, Mir Farzin Mashreghi, Alessio Mazzoni, James McCluskey, Henrik E. Mei, Fritz Melchers, Susanne Melzer, Dirk Mielenz, Leticia Monin, Lorenzo Moretta, Gabriele Multhoff, Luis Enrique Muñoz, Miguel Muñoz-Ruiz, Franziska Muscate, Ambra Natalini, Katrin Neumann, Lai Guan Ng, Antonia Niedobitek, Jana Niemz, Larissa Nogueira Almeida, Samuele Notarbartolo, Lennard Ostendorf, Laura J. Pallett, Amit A. Patel, Gulce Itir Percin, Giovanna Peruzzi, Marcello Pinti, A. Graham Pockley, Katharina Pracht, Immo Prinz, Irma Pujol-Autonell, Nadia Pulvirenti, Linda Quatrini, Kylie M. Quinn, Helena Radbruch, Hefin Rhys, Maria B. Rodrigo, Chiara Romagnani, Carina Saggau, Shimon Sakaguchi, Federica Sallusto, Lieke Sanderink, Inga Sandrock, Christine Schauer, Alexander Scheffold, Hans U. Scherer, Matthias Schiemann, Frank A. Schildberg, Kilian Schober, Janina Schoen, Wolfgang Schuh, Thomas Schüler, Axel R. Schulz, Sebastian Schulz, Julia Schulze, Sonia Simonetti, Jeeshan Singh, Katarzyna M. Sitnik, Regina Stark, Sarah Starossom, Christina Stehle, Franziska Szelinski, Leonard Tan, Attila Tarnok, Julia Tornack, Timothy I.M. Tree, Jasper J.P. van Beek, Willem van de Veen, Klaas van Gisbergen, Chiara Vasco, Nikita A. Verheyden, Anouk von Borstel, Kirsten A. Ward-Hartstonge, Klaus Warnatz, Claudia Waskow, Annika Wiedemann, Anneke Wilharm, James Wing, Oliver Wirz, Jens Wittner, Jennie H.M. Yang, Juhao Yang

^*Corresponding author for this work

462 Citations (Scopus)

Abstract

The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples with examples of flow cytometry applications in the context of autoimmune diseases, cancers as well as acute and chronic infectious diseases. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer-reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state-of-the-art handbook for basic and clinical researchers.

Original language	English
Journal	European Journal of Immunology
Volume	51
Issue number	12
Pages (from-to)	2708-3145
Number of pages	438
ISSN	0014-2980
DOIs	https://doi.org/10.1002/eji.202170126
Publication status	Published - 12.2021

Funding

Funders	Funder number
Fondazione Italiana per la Ricerca sul Cancro
Alexander Scheffold
Rebecca Cornelis
Carina Saggau
H2020 Marie Skłodowska-Curie Actions
Anouk von Borstel
V.7 Human plasma cells in multiple myeloma
Chiara Vasco
James Wing, Ilenia Cammarata
Jenny F. Kuehne, Christine S. Falk
Daniela Carolina Hernández
Ambra Natalini
Larissa Nogueira Almeida
Regina Stark
Rudolf Armin Manz
Sonia Simonetti
Petra Bacher
Fondazione Italiana per la Ricerca sul Cancro-Associazione Italiana per la Ricerca sul Cancro
Antonia Niedobitek
Horizon 2020
Fondazione Italiana per la Ricerca sul Cancro-Associazione Italiana per la Ricerca sul Cancro
Associazione Italiana per la Ricerca sul Cancro	IG20676
Shenzhen Gas Corporation	GA 2441/3-1
National Institutes of Health	19920, 21147, 5X1000 2018, MA 2273/14-1
Chinesisch-Deutsche Zentrum für Wissenschaftsförderung	C‐0072, SFB841, MA 2273/14‐1, HU 1714/10‐1
Not added	MR/V02423X/1
Deutsche Forschungsgemeinschaft	390873048, 197‐205, 389687267, EXC2151, BA 5132/1‐2, 252623821
Horizon 2020 Framework Programme	800924

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Areas and Centers

Academic Focus: Center for Infection and Inflammation Research (ZIEL)

DFG Research Classification Scheme

2.21-05 Immunology

Access to Document

10.1002/eji.202170126

Cite this

Cossarizza, A., Chang, H. D., Radbruch, A., Abrignani, S., Addo, R., Akdis, M., Andrä, I., Andreata, F., Annunziato, F., Arranz, E., Bacher, P., Bari, S., Barnaba, V., Barros-Martins, J., Baumjohann, D., Beccaria, C. G., Bernardo, D., Boardman, D. A., Borger, J., ... Yang, J. (2021). Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition). European Journal of Immunology, 51(12), 2708-3145. https://doi.org/10.1002/eji.202170126

@article{705d25d3ff724391aeb5207e3296055f,

title = "Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)",

abstract = "The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples with examples of flow cytometry applications in the context of autoimmune diseases, cancers as well as acute and chronic infectious diseases. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer-reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state-of-the-art handbook for basic and clinical researchers.",

author = "Andrea Cossarizza and Chang, \{Hyun Dong\} and Andreas Radbruch and Sergio Abrignani and Richard Addo and M{\"u}beccel Akdis and Immanuel Andr{\"a} and Francesco Andreata and Francesco Annunziato and Eduardo Arranz and Petra Bacher and Sudipto Bari and Vincenzo Barnaba and Joana Barros-Martins and Dirk Baumjohann and Beccaria, \{Cristian G.\} and David Bernardo and Boardman, \{Dominic A.\} and Jessica Borger and Chotima B{\"o}ttcher and Leonie Brockmann and Marie Burns and Busch, \{Dirk H.\} and Garth Cameron and Ilenia Cammarata and Antonino Cassotta and Yinshui Chang and Chirdo, \{Fernando Gabriel\} and Eleni Christakou and Luka {\v C}i{\v c}in-{\v S}ain and Laura Cook and Corbett, \{Alexandra J.\} and Rebecca Cornelis and Lorenzo Cosmi and Davey, \{Martin S.\} and \{De Biasi\}, Sara and \{De Simone\}, Gabriele and \{del Zotto\}, Genny and Michael Delacher and \{Di Rosa\}, Francesca and Santo, \{James Di\} and Andreas Diefenbach and Jun Dong and Thomas D{\"o}rner and Dress, \{Regine J.\} and Dutertre, \{Charles Antoine\} and Eckle, \{Sidonia B.G.\} and Pascale Eede and Maximilien Evrard and Falk, \{Christine S.\} and Markus Feuerer and Simon Fillatreau and Aida Fiz-Lopez and Marie Follo and Foulds, \{Gemma A.\} and Julia Fr{\"o}bel and Nicola Gagliani and Giovanni Galletti and Anastasia Gangaev and Natalio Garbi and Garrote, \{Jos{\'e} Antonio\} and Jens Geginat and Gherardin, \{Nicholas A.\} and Lara Gibellini and Florent Ginhoux and Godfrey, \{Dale I.\} and Paola Gruarin and Claudia Haftmann and Leo Hansmann and Harpur, \{Christopher M.\} and Hayday, \{Adrian C.\} and Guido Heine and Hern{\'a}ndez, \{Daniela Carolina\} and Martin Herrmann and Oliver Hoelsken and Qing Huang and Samuel Huber and Huber, \{Johanna E.\} and Jochen Huehn and Michael Hundemer and Hwang, \{William Y.K.\} and Matteo Iannacone and Ivison, \{Sabine M.\} and J{\"a}ck, \{Hans Martin\} and Jani, \{Peter K.\} and Baerbel Keller and Nina Kessler and Steven Ketelaars and Laura Knop and Jasmin Knopf and Koay, \{Hui Fern\} and Katja Kobow and Katharina Kriegsmann and H. Kristyanto and Andreas Krueger and Kuehne, \{Jenny F.\} and Heike Kunze-Schumacher and Pia Kvistborg and Immanuel Kwok and Daniela Latorre and Daniel Lenz and Levings, \{Megan K.\} and Lino, \{Andreia C.\} and Francesco Liotta and Long, \{Heather M.\} and Enrico Lugli and MacDonald, \{Katherine N.\} and Laura Maggi and Maini, \{Mala K.\} and Florian Mair and Calin Manta and Manz, \{Rudolf Armin\} and Mashreghi, \{Mir Farzin\} and Alessio Mazzoni and James McCluskey and Mei, \{Henrik E.\} and Fritz Melchers and Susanne Melzer and Dirk Mielenz and Leticia Monin and Lorenzo Moretta and Gabriele Multhoff and Mu{\~n}oz, \{Luis Enrique\} and Miguel Mu{\~n}oz-Ruiz and Franziska Muscate and Ambra Natalini and Katrin Neumann and Ng, \{Lai Guan\} and Antonia Niedobitek and Jana Niemz and Almeida, \{Larissa Nogueira\} and Samuele Notarbartolo and Lennard Ostendorf and Pallett, \{Laura J.\} and Patel, \{Amit A.\} and Percin, \{Gulce Itir\} and Giovanna Peruzzi and Marcello Pinti and Pockley, \{A. Graham\} and Katharina Pracht and Immo Prinz and Irma Pujol-Autonell and Nadia Pulvirenti and Linda Quatrini and Quinn, \{Kylie M.\} and Helena Radbruch and Hefin Rhys and Rodrigo, \{Maria B.\} and Chiara Romagnani and Carina Saggau and Shimon Sakaguchi and Federica Sallusto and Lieke Sanderink and Inga Sandrock and Christine Schauer and Alexander Scheffold and Scherer, \{Hans U.\} and Matthias Schiemann and Schildberg, \{Frank A.\} and Kilian Schober and Janina Schoen and Wolfgang Schuh and Thomas Sch{\"u}ler and Schulz, \{Axel R.\} and Sebastian Schulz and Julia Schulze and Sonia Simonetti and Jeeshan Singh and Sitnik, \{Katarzyna M.\} and Regina Stark and Sarah Starossom and Christina Stehle and Franziska Szelinski and Leonard Tan and Attila Tarnok and Julia Tornack and Tree, \{Timothy I.M.\} and \{van Beek\}, \{Jasper J.P.\} and \{van de Veen\}, Willem and \{van Gisbergen\}, Klaas and Chiara Vasco and Verheyden, \{Nikita A.\} and \{von Borstel\}, Anouk and Ward-Hartstonge, \{Kirsten A.\} and Klaus Warnatz and Claudia Waskow and Annika Wiedemann and Anneke Wilharm and James Wing and Oliver Wirz and Jens Wittner and Yang, \{Jennie H.M.\} and Juhao Yang",

note = "Publisher Copyright: {\textcopyright} 2021 Wiley-VCH GmbH",

year = "2021",

month = dec,

doi = "10.1002/eji.202170126",

language = "English",

volume = "51",

pages = "2708--3145",

journal = "European Journal of Immunology",

issn = "0014-2980",

publisher = "Wiley-VCH Verlag",

number = "12",

}

Cossarizza, A, Chang, HD, Radbruch, A, Abrignani, S, Addo, R, Akdis, M, Andrä, I, Andreata, F, Annunziato, F, Arranz, E, Bacher, P, Bari, S, Barnaba, V, Barros-Martins, J, Baumjohann, D, Beccaria, CG, Bernardo, D, Boardman, DA, Borger, J, Böttcher, C, Brockmann, L, Burns, M, Busch, DH, Cameron, G, Cammarata, I, Cassotta, A, Chang, Y, Chirdo, FG, Christakou, E, Čičin-Šain, L, Cook, L, Corbett, AJ, Cornelis, R, Cosmi, L, Davey, MS, De Biasi, S, De Simone, G, del Zotto, G, Delacher, M, Di Rosa, F, Santo, JD, Diefenbach, A, Dong, J, Dörner, T, Dress, RJ, Dutertre, CA, Eckle, SBG, Eede, P, Evrard, M, Falk, CS, Feuerer, M, Fillatreau, S, Fiz-Lopez, A, Follo, M, Foulds, GA, Fröbel, J, Gagliani, N, Galletti, G, Gangaev, A, Garbi, N, Garrote, JA, Geginat, J, Gherardin, NA, Gibellini, L, Ginhoux, F, Godfrey, DI, Gruarin, P, Haftmann, C, Hansmann, L, Harpur, CM, Hayday, AC, Heine, G, Hernández, DC, Herrmann, M, Hoelsken, O, Huang, Q, Huber, S, Huber, JE, Huehn, J, Hundemer, M, Hwang, WYK, Iannacone, M, Ivison, SM, Jäck, HM, Jani, PK, Keller, B, Kessler, N, Ketelaars, S, Knop, L, Knopf, J, Koay, HF, Kobow, K, Kriegsmann, K, Kristyanto, H, Krueger, A, Kuehne, JF, Kunze-Schumacher, H, Kvistborg, P, Kwok, I, Latorre, D, Lenz, D, Levings, MK, Lino, AC, Liotta, F, Long, HM, Lugli, E, MacDonald, KN, Maggi, L, Maini, MK, Mair, F, Manta, C, Manz, RA, Mashreghi, MF, Mazzoni, A, McCluskey, J, Mei, HE, Melchers, F, Melzer, S, Mielenz, D, Monin, L, Moretta, L, Multhoff, G, Muñoz, LE, Muñoz-Ruiz, M, Muscate, F, Natalini, A, Neumann, K, Ng, LG, Niedobitek, A, Niemz, J, Almeida, LN, Notarbartolo, S, Ostendorf, L, Pallett, LJ, Patel, AA, Percin, GI, Peruzzi, G, Pinti, M, Pockley, AG, Pracht, K, Prinz, I, Pujol-Autonell, I, Pulvirenti, N, Quatrini, L, Quinn, KM, Radbruch, H, Rhys, H, Rodrigo, MB, Romagnani, C, Saggau, C, Sakaguchi, S, Sallusto, F, Sanderink, L, Sandrock, I, Schauer, C, Scheffold, A, Scherer, HU, Schiemann, M, Schildberg, FA, Schober, K, Schoen, J, Schuh, W, Schüler, T, Schulz, AR, Schulz, S, Schulze, J, Simonetti, S, Singh, J, Sitnik, KM, Stark, R, Starossom, S, Stehle, C, Szelinski, F, Tan, L, Tarnok, A, Tornack, J, Tree, TIM, van Beek, JJP, van de Veen, W, van Gisbergen, K, Vasco, C, Verheyden, NA, von Borstel, A, Ward-Hartstonge, KA, Warnatz, K, Waskow, C, Wiedemann, A, Wilharm, A, Wing, J, Wirz, O, Wittner, J, Yang, JHM & Yang, J 2021, 'Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)', European Journal of Immunology, vol. 51, no. 12, pp. 2708-3145. https://doi.org/10.1002/eji.202170126

TY - JOUR

T1 - Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

AU - Cossarizza, Andrea

AU - Chang, Hyun Dong

AU - Radbruch, Andreas

AU - Abrignani, Sergio

AU - Addo, Richard

AU - Akdis, Mübeccel

AU - Andrä, Immanuel

AU - Andreata, Francesco

AU - Annunziato, Francesco

AU - Arranz, Eduardo

AU - Bacher, Petra

AU - Bari, Sudipto

AU - Barnaba, Vincenzo

AU - Barros-Martins, Joana

AU - Baumjohann, Dirk

AU - Beccaria, Cristian G.

AU - Bernardo, David

AU - Boardman, Dominic A.

AU - Borger, Jessica

AU - Böttcher, Chotima

AU - Brockmann, Leonie

AU - Burns, Marie

AU - Busch, Dirk H.

AU - Cameron, Garth

AU - Cammarata, Ilenia

AU - Cassotta, Antonino

AU - Chang, Yinshui

AU - Chirdo, Fernando Gabriel

AU - Christakou, Eleni

AU - Čičin-Šain, Luka

AU - Cook, Laura

AU - Corbett, Alexandra J.

AU - Cornelis, Rebecca

AU - Cosmi, Lorenzo

AU - Davey, Martin S.

AU - De Biasi, Sara

AU - De Simone, Gabriele

AU - del Zotto, Genny

AU - Delacher, Michael

AU - Di Rosa, Francesca

AU - Santo, James Di

AU - Diefenbach, Andreas

AU - Dong, Jun

AU - Dörner, Thomas

AU - Dress, Regine J.

AU - Dutertre, Charles Antoine

AU - Eckle, Sidonia B.G.

AU - Eede, Pascale

AU - Evrard, Maximilien

AU - Falk, Christine S.

AU - Feuerer, Markus

AU - Fillatreau, Simon

AU - Fiz-Lopez, Aida

AU - Follo, Marie

AU - Foulds, Gemma A.

AU - Fröbel, Julia

AU - Gagliani, Nicola

AU - Galletti, Giovanni

AU - Gangaev, Anastasia

AU - Garbi, Natalio

AU - Garrote, José Antonio

AU - Geginat, Jens

AU - Gherardin, Nicholas A.

AU - Gibellini, Lara

AU - Ginhoux, Florent

AU - Godfrey, Dale I.

AU - Gruarin, Paola

AU - Haftmann, Claudia

AU - Hansmann, Leo

AU - Harpur, Christopher M.

AU - Hayday, Adrian C.

AU - Heine, Guido

AU - Hernández, Daniela Carolina

AU - Herrmann, Martin

AU - Hoelsken, Oliver

AU - Huang, Qing

AU - Huber, Samuel

AU - Huber, Johanna E.

AU - Huehn, Jochen

AU - Hundemer, Michael

AU - Hwang, William Y.K.

AU - Iannacone, Matteo

AU - Ivison, Sabine M.

AU - Jäck, Hans Martin

AU - Jani, Peter K.

AU - Keller, Baerbel

AU - Kessler, Nina

AU - Ketelaars, Steven

AU - Knop, Laura

AU - Knopf, Jasmin

AU - Koay, Hui Fern

AU - Kobow, Katja

AU - Kriegsmann, Katharina

AU - Kristyanto, H.

AU - Krueger, Andreas

AU - Kuehne, Jenny F.

AU - Kunze-Schumacher, Heike

AU - Kvistborg, Pia

AU - Kwok, Immanuel

AU - Latorre, Daniela

AU - Lenz, Daniel

AU - Levings, Megan K.

AU - Lino, Andreia C.

AU - Liotta, Francesco

AU - Long, Heather M.

AU - Lugli, Enrico

AU - MacDonald, Katherine N.

AU - Maggi, Laura

AU - Maini, Mala K.

AU - Mair, Florian

AU - Manta, Calin

AU - Manz, Rudolf Armin

AU - Mashreghi, Mir Farzin

AU - Mazzoni, Alessio

AU - McCluskey, James

AU - Mei, Henrik E.

AU - Melchers, Fritz

AU - Melzer, Susanne

AU - Mielenz, Dirk

AU - Monin, Leticia

AU - Moretta, Lorenzo

AU - Multhoff, Gabriele

AU - Muñoz, Luis Enrique

AU - Muñoz-Ruiz, Miguel

AU - Muscate, Franziska

AU - Natalini, Ambra

AU - Neumann, Katrin

AU - Ng, Lai Guan

AU - Niedobitek, Antonia

AU - Niemz, Jana

AU - Almeida, Larissa Nogueira

AU - Notarbartolo, Samuele

AU - Ostendorf, Lennard

AU - Pallett, Laura J.

AU - Patel, Amit A.

AU - Percin, Gulce Itir

AU - Peruzzi, Giovanna

AU - Pinti, Marcello

AU - Pockley, A. Graham

AU - Pracht, Katharina

AU - Prinz, Immo

AU - Pujol-Autonell, Irma

AU - Pulvirenti, Nadia

AU - Quatrini, Linda

AU - Quinn, Kylie M.

AU - Radbruch, Helena

AU - Rhys, Hefin

AU - Rodrigo, Maria B.

AU - Romagnani, Chiara

AU - Saggau, Carina

AU - Sakaguchi, Shimon

AU - Sallusto, Federica

AU - Sanderink, Lieke

AU - Sandrock, Inga

AU - Schauer, Christine

AU - Scheffold, Alexander

AU - Scherer, Hans U.

AU - Schiemann, Matthias

AU - Schildberg, Frank A.

AU - Schober, Kilian

AU - Schoen, Janina

AU - Schuh, Wolfgang

AU - Schüler, Thomas

AU - Schulz, Axel R.

AU - Schulz, Sebastian

AU - Schulze, Julia

AU - Simonetti, Sonia

AU - Singh, Jeeshan

AU - Sitnik, Katarzyna M.

AU - Stark, Regina

AU - Starossom, Sarah

AU - Stehle, Christina

AU - Szelinski, Franziska

AU - Tan, Leonard

AU - Tarnok, Attila

AU - Tornack, Julia

AU - Tree, Timothy I.M.

AU - van Beek, Jasper J.P.

AU - van de Veen, Willem

AU - van Gisbergen, Klaas

AU - Vasco, Chiara

AU - Verheyden, Nikita A.

AU - von Borstel, Anouk

AU - Ward-Hartstonge, Kirsten A.

AU - Warnatz, Klaus

AU - Waskow, Claudia

AU - Wiedemann, Annika

AU - Wilharm, Anneke

AU - Wing, James

AU - Wirz, Oliver

AU - Wittner, Jens

AU - Yang, Jennie H.M.

AU - Yang, Juhao

PY - 2021/12

Y1 - 2021/12

N2 - The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples with examples of flow cytometry applications in the context of autoimmune diseases, cancers as well as acute and chronic infectious diseases. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer-reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state-of-the-art handbook for basic and clinical researchers.

AB - The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples with examples of flow cytometry applications in the context of autoimmune diseases, cancers as well as acute and chronic infectious diseases. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer-reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state-of-the-art handbook for basic and clinical researchers.

UR - https://www.scopus.com/pages/publications/85119620202

U2 - 10.1002/eji.202170126

DO - 10.1002/eji.202170126

M3 - Journal articles

C2 - 34910301

AN - SCOPUS:85119620202

SN - 0014-2980

VL - 51

SP - 2708

EP - 3145

JO - European Journal of Immunology

JF - European Journal of Immunology

IS - 12

ER -

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

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