Growth-Modulatory effects of heparin and vegf165 on the Choriocarcinoma Cell-line JEG-3 and its expression of heparanase

Background: Expression of heparanase (HPSE) in tumor cells is strongly associated with invasion, metastasis and angiogenesis. It also plays a key role during pregnancy, in processes of implantation as well as placentation. Vascular endothelial growth factor (VEGF) and heparin are known to alter HPSE expression, with heparin given prophylactically to women with a history of placenta-mediated complications in subsequent pregnancies. Materials and Methods: We examined the growth-modulatory effects of different concentrations of heparin and VEGF on the choriocarcinoma cell-line JEG-3 and the expression of heparanase under VEGF and heparin by proliferation assays, PCR, and western blot. Results: Proliferation of JEG-3 cells was induced by heparin in a dosedependent manner, whereas highly concentrated VEGF led to a decreased cell proliferation. Both agents did not influence the HPSE-expression. Conclusion: The presumed pregnancyprotecting effects of heparin may partially be due to an increase of trophoblast proliferation and not via regulation of HPSE expression.