Generierung und konservierung von keimzellen. Von der in-vitro-maturation bis zur oozytenvitrifikation

Translated title of the contribution: Generation and conservation of stem cells. From in vitro maturation to vitrification of oocytes

M. Montag*, V. Isachenko, E. Isachenko, M. Von Wolff, S. Von Otte, A. Schultze-Mosgau, S. Al-Hasani

*Corresponding author for this work

Abstract

In vitro maturation (IVM) is a technique which allows the maturation of oocytes from the germinal vesicle stage up to the stage of the fertilization-competent metaphase-II oocytes. Immature oocytes are primarily retrieved from small antral follicles. Their successful maturation is usually documented by formation of the first polar body as an indicator of completion of the first meiotic division. The quality of in vitro matured oocytes can now be judged by polarisation microscopy. This technique allows better quality assessment and is hence a unique instrument for optimising existing IVM protocols. In combination with the now realistic option of successful cryopreservation of mature and immature oocytes by the technique of vitrification, IVM will soon enter new fields of application.

Translated title of the contributionGeneration and conservation of stem cells. From in vitro maturation to vitrification of oocytes
Original languageGerman
JournalGynakologische Endokrinologie
Volume4
Issue number4
Pages (from-to)205-210
Number of pages6
ISSN1610-2894
DOIs
Publication statusPublished - 11.2006

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