Generation of recombinant Norovirus-like particles (VLP) in the human endothelial kidney cell line 293T

S. Taube, A. Kurth, E. Schreier*

*Corresponding author for this work
20 Citations (Scopus)

Abstract

Noroviruses (NVs) are the major cause of non-bacterial outbreaks of gastroenteritis. Here we report a new alternative system to generate recombinant NV virus-like particles (rNV-VLP) in a human endothelial kidney cell line (HEK). Transfecting HEK-293T cells with an expression vector coding for the ORF-2 gene lead to the expression of the viral structural protein VP1 which spontaneously assembled into virus-like particles (VLP), as shown by electron microscopy. The transfected cells did not show a cytopathic effect and released rNV-VLP into the culture medium. The HEK-293T cell derived particles were morphologically indistinguishable to the rNV-VLP produced from baculovirus and the Venezuelan equine encephalitis virus (VEE)-replicon. The produced particles were stable for at least 2.5 months at 4°C.

Original languageEnglish
JournalArchives of Virology
Volume150
Issue number7
Pages (from-to)1425-1431
Number of pages7
ISSN0304-8608
DOIs
Publication statusPublished - 01.07.2005

Research Areas and Centers

  • Academic Focus: Center for Infection and Inflammation Research (ZIEL)

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