G protein-coupled receptor 43 is essential for neutrophil recruitment during intestinal inflammation

Christian Sina, Olga Gavrilova, Matti Fórster, Andreas Till, Stefanie Derer, Friederike Hildebrand, Bjórn Raabe, Athena Chalaris, Júrgen Scheller, Ateequr Rehmann, Andre Franke, Stephan Ott, Robert Hásler, Susanna Nikolaus, Ulrich R. Fólsch, Stefan Rose-John, Hui Ping Jiang, Jun Li, Stefan Schreiber*, Philip Rosenstiel

*Corresponding author for this work
217 Citations (Scopus)


Molecular danger signals attract neutrophilic granulocytes (polymorphonuclear leukocytes (PMNs)) to sites of infection. The G protein-coupled receptor (GPR) 43 recognizes propionate and butyrate and is abundantly expressed on PMNs. The functional role of GPR43 activation for in vivo orchestration of immune response is unclear. We examined dextrane sodium sulfate (DSS)-induced acute and chronic intestinal inflammatory response in wild-type and Gpr43-deficient mice. The severity of colonic inflammation was assessed by clinical signs, histological scoring, and cytokine production. Chemotaxis of wild-type and Gpr43-deficient PMNs was assessed through transwell cell chemotactic assay. A reduced invasion of PMNs and increased mortality due to septic complications were observed in acute DSS colitis. In chronic DSS colitis, Gpr43-/- animals showed diminished PMN intestinal migration, but protection against inflammatory tissue destruction. No significant difference in PMN migration and cytokine secretion was detected in a sterile inflammatory model. Ex vivo experiments show that GPR43-induced migration is dependent on activation of the protein kinase p38α, and that this signal acts in cooperation with the chemotactic cytokine keratinocyte chemoattractant. Interestingly, shedding of L-selectin in response to propionate and butyrate was compromised in Gpr43-/- mice. These results indicate a critical role for GPR43-mediated recruitment of PMNs in containing intestinal bacterial translocation, yet also emphasize the bipotential role of PMNs in mediating tissue destruction in chronic intestinal inflammation.

Original languageEnglish
JournalJournal of Immunology
Issue number11
Pages (from-to)7514-7522
Number of pages9
Publication statusPublished - 01.12.2009


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