Functional testing in a mouse stroke model induced by occlusion of the distal middle cerebral artery

Judith Lubjuhn, Alexandra Gastens, Gertrud von Wilpert, Panagiotis Bargiotas, Oliver Herrmann, Sasidhar Murikinati, Tamer Rabie, Hugo Marti, Ivo Amende, Tom G. Hampton, Markus Schwaninger*

*Corresponding author for this work
47 Citations (Scopus)


Reducing post-stroke disability is the major goal of stroke therapy. Consequently, functional testing is essential in experimental stroke studies to increase the predictive value of animal models. We used several sensory and motor tests to assess functional disability in a mouse model of permanent distal middle cerebral artery occlusion (pdMCAO) that induced mainly cortical infarcts. Gait dynamics were transiently disturbed after pdMCAO as measured by different analysis techniques. Stance and brake duration were shorter after pdMCAO. Consistent with sensory and motor deficits the latency to move was prolonged up to 14 days after pdMCAO and the performance in the corner test and handedness were affected on day 1 or 2 after pdMCAO. Heart rate was decreased and heart rate variability were increased after pdMCAO indicating sympathetic-parasympathetic imbalance. In summary, pdMCAO-induced cortical infarcts lead to clinically relevant sensory, motor and cardiac autonomic dysfunction in mice. The present study provides a basis to explore the potential of functional testing for neuroprotection and neuroregeneration after stroke.

Original languageEnglish
JournalJournal of Neuroscience Methods
Issue number1
Pages (from-to)95-103
Number of pages9
Publication statusPublished - 30.10.2009

Research Areas and Centers

  • Academic Focus: Center for Brain, Behavior and Metabolism (CBBM)


Dive into the research topics of 'Functional testing in a mouse stroke model induced by occlusion of the distal middle cerebral artery'. Together they form a unique fingerprint.

Cite this