Functional expression of a human C5a receptor clone in Xenopus oocytes requires additional RNA

Beate Kroll; Monica Emde; Andreas Jeromin; Lothar Penner; Gerhard Rechkemmer; Titus Kretzschmar; Andreas Klos; Jörg Köhl; Wilfried Bautsch

doi:10.1016/0014-5793(91)81285-G

Functional expression of a human C5a receptor clone in Xenopus oocytes requires additional RNA

Beate Kroll, Monica Emde, Andreas Jeromin, Lothar Penner, Gerhard Rechkemmer, Titus Kretzschmar, Andreas Klos, Jörg Köhl, Wilfried Bautsch^*

^*Corresponding author for this work

Institute of Systemic Inflamation Research

12 Citations (Scopus)

Abstract

cRNA from a PCR-generated C5aR clone was prepared by in vitro transcription and microinjected into Xenopus laevis oocytes. Ligand-induced whole cell current could be detected after co-injection of cRNA for the C5aR with total RNA of the unstimulated U937 cell line, but not with either of the components injected alone. These data clearly demonstrate an absolute requirement of the C5aR for an additional human factor to become functionally expressed in Xenopus oocytes.

Original language	English
Journal	FEBS Letters
Volume	291
Issue number	2
Pages (from-to)	208-210
Number of pages	3
ISSN	0014-5793
DOIs	https://doi.org/10.1016/0014-5793(91)81285-G
Publication status	Published - 21.10.1991

Funding

Ackrtorv/cdgememW ccorcl~ally thank 5 Bitter-Sucrmannfo r contl-nuous supporta nd many helpful dIscussIonsT his work was supported by grants of the DFG SonderforschungsbereIc2h4 4‘Chromsche Entzdndungen’a nd the Cystrc Flbrosls Foundation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1016/0014-5793(91)81285-G

Cite this

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title = "Functional expression of a human C5a receptor clone in Xenopus oocytes requires additional RNA",

abstract = "cRNA from a PCR-generated C5aR clone was prepared by in vitro transcription and microinjected into Xenopus laevis oocytes. Ligand-induced whole cell current could be detected after co-injection of cRNA for the C5aR with total RNA of the unstimulated U937 cell line, but not with either of the components injected alone. These data clearly demonstrate an absolute requirement of the C5aR for an additional human factor to become functionally expressed in Xenopus oocytes.",

author = "Beate Kroll and Monica Emde and Andreas Jeromin and Lothar Penner and Gerhard Rechkemmer and Titus Kretzschmar and Andreas Klos and J{\"o}rg K{\"o}hl and Wilfried Bautsch",

year = "1991",

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doi = "10.1016/0014-5793(91)81285-G",

language = "English",

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T1 - Functional expression of a human C5a receptor clone in Xenopus oocytes requires additional RNA

AU - Kroll, Beate

AU - Emde, Monica

AU - Jeromin, Andreas

AU - Penner, Lothar

AU - Rechkemmer, Gerhard

AU - Kretzschmar, Titus

AU - Klos, Andreas

AU - Köhl, Jörg

AU - Bautsch, Wilfried

PY - 1991/10/21

Y1 - 1991/10/21

N2 - cRNA from a PCR-generated C5aR clone was prepared by in vitro transcription and microinjected into Xenopus laevis oocytes. Ligand-induced whole cell current could be detected after co-injection of cRNA for the C5aR with total RNA of the unstimulated U937 cell line, but not with either of the components injected alone. These data clearly demonstrate an absolute requirement of the C5aR for an additional human factor to become functionally expressed in Xenopus oocytes.

AB - cRNA from a PCR-generated C5aR clone was prepared by in vitro transcription and microinjected into Xenopus laevis oocytes. Ligand-induced whole cell current could be detected after co-injection of cRNA for the C5aR with total RNA of the unstimulated U937 cell line, but not with either of the components injected alone. These data clearly demonstrate an absolute requirement of the C5aR for an additional human factor to become functionally expressed in Xenopus oocytes.

UR - https://www.scopus.com/pages/publications/0026049699

U2 - 10.1016/0014-5793(91)81285-G

DO - 10.1016/0014-5793(91)81285-G

M3 - Journal articles

C2 - 1718782

AN - SCOPUS:0026049699

SN - 0014-5793

VL - 291

SP - 208

EP - 210

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Functional expression of a human C5a receptor clone in Xenopus oocytes requires additional RNA

Abstract

Funding

UN SDGs

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