Functional expression of a human C5a receptor clone in Xenopus oocytes requires additional RNA

Beate Kroll; Monica Emde; Andreas Jeromin; Lothar Penner; Gerhard Rechkemmer; Titus Kretzschmar; Andreas Klos; Jörg Köhl; Wilfried Bautsch

doi:10.1016/0014-5793(91)81285-G

Functional expression of a human C5a receptor clone in Xenopus oocytes requires additional RNA

Beate Kroll, Monica Emde, Andreas Jeromin, Lothar Penner, Gerhard Rechkemmer, Titus Kretzschmar, Andreas Klos, Jörg Köhl, Wilfried Bautsch^*

^*Corresponding author for this work

Institute of Systemic Inflamation Research

9 Citations (Scopus)

Abstract

cRNA from a PCR-generated C5aR clone was prepared by in vitro transcription and microinjected into Xenopus laevis oocytes. Ligand-induced whole cell current could be detected after co-injection of cRNA for the C5aR with total RNA of the unstimulated U937 cell line, but not with either of the components injected alone. These data clearly demonstrate an absolute requirement of the C5aR for an additional human factor to become functionally expressed in Xenopus oocytes.

Original language	English
Journal	FEBS Letters
Volume	291
Issue number	2
Pages (from-to)	208-210
Number of pages	3
ISSN	0014-5793
DOIs	https://doi.org/10.1016/0014-5793(91)81285-G
Publication status	Published - 21.10.1991

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10.1016/0014-5793(91)81285-G

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abstract = "cRNA from a PCR-generated C5aR clone was prepared by in vitro transcription and microinjected into Xenopus laevis oocytes. Ligand-induced whole cell current could be detected after co-injection of cRNA for the C5aR with total RNA of the unstimulated U937 cell line, but not with either of the components injected alone. These data clearly demonstrate an absolute requirement of the C5aR for an additional human factor to become functionally expressed in Xenopus oocytes.",

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AU - Kroll, Beate

AU - Emde, Monica

AU - Jeromin, Andreas

AU - Penner, Lothar

AU - Rechkemmer, Gerhard

AU - Kretzschmar, Titus

AU - Klos, Andreas

AU - Köhl, Jörg

AU - Bautsch, Wilfried

PY - 1991/10/21

Y1 - 1991/10/21

N2 - cRNA from a PCR-generated C5aR clone was prepared by in vitro transcription and microinjected into Xenopus laevis oocytes. Ligand-induced whole cell current could be detected after co-injection of cRNA for the C5aR with total RNA of the unstimulated U937 cell line, but not with either of the components injected alone. These data clearly demonstrate an absolute requirement of the C5aR for an additional human factor to become functionally expressed in Xenopus oocytes.

AB - cRNA from a PCR-generated C5aR clone was prepared by in vitro transcription and microinjected into Xenopus laevis oocytes. Ligand-induced whole cell current could be detected after co-injection of cRNA for the C5aR with total RNA of the unstimulated U937 cell line, but not with either of the components injected alone. These data clearly demonstrate an absolute requirement of the C5aR for an additional human factor to become functionally expressed in Xenopus oocytes.

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SP - 208

EP - 210

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Functional expression of a human C5a receptor clone in Xenopus oocytes requires additional RNA

Abstract

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