Functional alteration of myeloid dendritic cells through head and neck cancer

Carsten P. Brocks, Ralph Pries, Henning Frenzel, Martin Ernst, Peter Schlenke, Barbara Wollenberg*

*Corresponding author for this work
10 Citations (Scopus)


Background: In head and neck squamous cell carcinoma (HNSCC), the secretion of various immunosuppressive mediators contributes to large-scale effects on the immune functions. The influence of HNSCC on various cellular functions of human myeloid dendritic cells (MDC) was analyzed in this work. Materials and Methods: MDCs were isolated from peripheral blood by 'magnetic bead separation'. Cellular functions were analyzed using flow cytometry, migration- and ELISPOT assays as well as cytokine detection assays. Results: HNSCC massively affects MDCs to induce effective T-cell responses. Analysis of MDC migration and cytokine secretion revealed that HNSCC triggers the production of tumor-promoting and immunosuppressive cytokines IL-1 and IL-10 and results in an increased MDC migration activity. CpG-ODN is able to act contradictory to HNSCC. Conclusion: HNSCC is able to modulate various functions of human MDCs. CpG-ODN is suggested as a potential immunostimulatory agent of human MDC.

Original languageEnglish
JournalAnticancer Research
Issue number2
Pages (from-to)817-824
Number of pages8
Publication statusPublished - 03.2007


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