Functional alteration of myeloid dendritic cells through head and neck cancer

Carsten P. Brocks; Ralph Pries; Henning Frenzel; Martin Ernst; Peter Schlenke; Barbara Wollenberg

Functional alteration of myeloid dendritic cells through head and neck cancer

Carsten P. Brocks, Ralph Pries, Henning Frenzel, Martin Ernst, Peter Schlenke, Barbara Wollenberg^*

^*Corresponding author for this work

Airway Research Center North

10 Citations (Scopus)

Abstract

Background: In head and neck squamous cell carcinoma (HNSCC), the secretion of various immunosuppressive mediators contributes to large-scale effects on the immune functions. The influence of HNSCC on various cellular functions of human myeloid dendritic cells (MDC) was analyzed in this work. Materials and Methods: MDCs were isolated from peripheral blood by 'magnetic bead separation'. Cellular functions were analyzed using flow cytometry, migration- and ELISPOT assays as well as cytokine detection assays. Results: HNSCC massively affects MDCs to induce effective T-cell responses. Analysis of MDC migration and cytokine secretion revealed that HNSCC triggers the production of tumor-promoting and immunosuppressive cytokines IL-1 and IL-10 and results in an increased MDC migration activity. CpG-ODN is able to act contradictory to HNSCC. Conclusion: HNSCC is able to modulate various functions of human MDCs. CpG-ODN is suggested as a potential immunostimulatory agent of human MDC.

Original language	English
Journal	Anticancer Research
Volume	27
Issue number	2
Pages (from-to)	817-824
Number of pages	8
ISSN	0250-7005
Publication status	Published - 03.2007

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title = "Functional alteration of myeloid dendritic cells through head and neck cancer",

abstract = "Background: In head and neck squamous cell carcinoma (HNSCC), the secretion of various immunosuppressive mediators contributes to large-scale effects on the immune functions. The influence of HNSCC on various cellular functions of human myeloid dendritic cells (MDC) was analyzed in this work. Materials and Methods: MDCs were isolated from peripheral blood by 'magnetic bead separation'. Cellular functions were analyzed using flow cytometry, migration- and ELISPOT assays as well as cytokine detection assays. Results: HNSCC massively affects MDCs to induce effective T-cell responses. Analysis of MDC migration and cytokine secretion revealed that HNSCC triggers the production of tumor-promoting and immunosuppressive cytokines IL-1 and IL-10 and results in an increased MDC migration activity. CpG-ODN is able to act contradictory to HNSCC. Conclusion: HNSCC is able to modulate various functions of human MDCs. CpG-ODN is suggested as a potential immunostimulatory agent of human MDC.",

author = "Brocks, {Carsten P.} and Ralph Pries and Henning Frenzel and Martin Ernst and Peter Schlenke and Barbara Wollenberg",

year = "2007",

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language = "English",

volume = "27",

pages = "817--824",

journal = "Anticancer Research",

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TY - JOUR

T1 - Functional alteration of myeloid dendritic cells through head and neck cancer

AU - Brocks, Carsten P.

AU - Pries, Ralph

AU - Frenzel, Henning

AU - Ernst, Martin

AU - Schlenke, Peter

AU - Wollenberg, Barbara

PY - 2007/3

Y1 - 2007/3

N2 - Background: In head and neck squamous cell carcinoma (HNSCC), the secretion of various immunosuppressive mediators contributes to large-scale effects on the immune functions. The influence of HNSCC on various cellular functions of human myeloid dendritic cells (MDC) was analyzed in this work. Materials and Methods: MDCs were isolated from peripheral blood by 'magnetic bead separation'. Cellular functions were analyzed using flow cytometry, migration- and ELISPOT assays as well as cytokine detection assays. Results: HNSCC massively affects MDCs to induce effective T-cell responses. Analysis of MDC migration and cytokine secretion revealed that HNSCC triggers the production of tumor-promoting and immunosuppressive cytokines IL-1 and IL-10 and results in an increased MDC migration activity. CpG-ODN is able to act contradictory to HNSCC. Conclusion: HNSCC is able to modulate various functions of human MDCs. CpG-ODN is suggested as a potential immunostimulatory agent of human MDC.

AB - Background: In head and neck squamous cell carcinoma (HNSCC), the secretion of various immunosuppressive mediators contributes to large-scale effects on the immune functions. The influence of HNSCC on various cellular functions of human myeloid dendritic cells (MDC) was analyzed in this work. Materials and Methods: MDCs were isolated from peripheral blood by 'magnetic bead separation'. Cellular functions were analyzed using flow cytometry, migration- and ELISPOT assays as well as cytokine detection assays. Results: HNSCC massively affects MDCs to induce effective T-cell responses. Analysis of MDC migration and cytokine secretion revealed that HNSCC triggers the production of tumor-promoting and immunosuppressive cytokines IL-1 and IL-10 and results in an increased MDC migration activity. CpG-ODN is able to act contradictory to HNSCC. Conclusion: HNSCC is able to modulate various functions of human MDCs. CpG-ODN is suggested as a potential immunostimulatory agent of human MDC.

UR - http://www.scopus.com/inward/record.url?scp=34247118386&partnerID=8YFLogxK

M3 - Journal articles

C2 - 17465207

AN - SCOPUS:34247118386

SN - 0250-7005

VL - 27

SP - 817

EP - 824

JO - Anticancer Research

JF - Anticancer Research

IS - 2

ER -

Functional alteration of myeloid dendritic cells through head and neck cancer

Abstract

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