The analysis of time lapse data becomes a more and more important tool in (stem) cell biology, as this method enables a marker free analysis of cell populations over time and allows for the reconstruction of genealogical trees; these information can help to understand the mechanisms behind cell proliferation and differentiation. However, the crucial steps in a cell tracking algorithm (cell segmentation, cell tracking and mitosis detection) have to be performed with a high trustworthiness to find the correct lineage of each cell. Here, we present a novel cell tracking algorithm that combines microscopical images with different contrast mechanisms: first, oblique illumination is used to segment and track cells; second, phase contrast microscopy is used to detect the most critical events, the mitosis. The combination of both detection results in a more robust cell tracking. For evaluation, the free reference data CeTReS is used.

Original languageEnglish
Title of host publication9th IEEE International Symposium on Biomedical Imaging (ISBI) 2012
Number of pages4
Publication date12.07.2012
Article number12864349
ISBN (Print)978-1-4577-1857-1
ISBN (Electronic)978-1-4577-1858-8
Publication statusPublished - 12.07.2012
EventIEEE International Symposium on Biomedical Imaging (ISBI) 2012 - Centre Convencions International Barcelona (CCIB), Barcelona, Spain
Duration: 02.05.201205.05.2012


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