Abstract
Type 2 diabetes mellitus (T2DM) adversely affects the essential characteristics of adipose tissue-derived mesenchymal stem cells (AdMSCs). Given that T2DM is associated with an altered serum free fatty acid (FFA) profile, we examined whether diabetic serum FFAs influence the viability, differentiation, and fatty acid composition of the major lipid fractions of human AdMSCs in vitro. Serum FFAs were isolated from 7 diabetic and 10 healthy nondiabetic female individuals. AdMSCs were cultured and differentiated into primordial germ cell-like cells (PGCLCs) in the presence of either diabetic or nondiabetic FFAs. Cell viability was assessed using trypan blue staining. Cell differentiation was evaluated by measuring the PGCLC transcriptional markers Blimp1 and Stella. Lipid fractionation and fatty acid quantification were performed using thin-layer chromatography and gas–liquid chromatography, respectively. Both diabetic and nondiabetic FFAs significantly reduced the viability of PGCLCs. The gene expression of both differentiation markers was significantly lower in cells exposed to diabetic FFAs than in those treated with nondiabetic FFAs. Saturated fatty acids were significantly increased and linoleic acid was significantly decreased in the cellular phospholipid fraction after exposure to diabetic FFAs. In contrast, monounsaturated fatty acids were reduced and linoleic acid was elevated in the cellular triglyceride fraction in response to diabetic FFAs. Such an altered serum FFA profile in patients with T2DM reduces the proliferation and differentiation potential of AdMSCs, presumably due to the aberrant distribution of fatty acids into cell phospholipids and triglycerides. Graphical Abstract: [Figure not available: see fulltext.].
| Original language | English |
|---|---|
| Journal | Applied Biochemistry and Biotechnology |
| Volume | 195 |
| Issue number | 5 |
| Pages (from-to) | 3011-3026 |
| Number of pages | 16 |
| ISSN | 0273-2289 |
| DOIs | |
| Publication status | Published - 05.2023 |
Funding
This work was supported by grants from Endocrine Research Center, Tabriz University of Medical Sciences and Tabriz University of Medical Sciences (numbers [59420] and [66852]) to AM. The first author is supported by fellowship programs from the Alexander von Humboldt Foundation. The graphical designs were created with BioRender.com.
| Funders | Funder number |
|---|---|
| Endocrine Research Center, Tabriz University of Medical Sciences and Tabriz University of Medical Sciences | 66852, 59420 |
| Alexander von Humboldt Foundation | |
| Research Center for Infectious Diseases and Tropical Medicine, Tabriz University of Medical Sciences | |
| Drug Applied Research Center, Tabriz University of Medical Sciences | |
| Tabriz University of Medical Sciences | |
| Biotechnology Research Center, Tabriz University of Medical Sciences | |
| Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences | |
| Student Research Committee, Tabriz University of Medical Sciences |
Research Areas and Centers
- Centers: Center for Pre-Implantation Diagnostics (PID)
