TY - JOUR
T1 - Fractalkine and apoptotic/anti-apoptotic markers in granulosa cells of women with polycystic ovarian syndrome
AU - Raei Sadigh, Aydin
AU - Darabi, Masoud
AU - Salmassi, Ali
AU - Hamdi, Kobra
AU - Farzadi, Laya
AU - Ghasemzadeh, Aliye
AU - Fattahi, Amir
AU - Nouri, Mohammad
N1 - Funding Information:
This study was funded by the Stem Cell Research Center, Tabriz University of Medical Sciences [Grant Number: 57778]. Acknowledgments
Funding Information:
We thank staff of Al-Zahra Hospital of Tabriz and Milad Fertility Center for providing the patients. Some of the data included are part of the M.Sc. thesis of Aydin Raei Sadigh.
Publisher Copyright:
© 2020, Springer Nature B.V.
PY - 2020/5/1
Y1 - 2020/5/1
N2 - Owing to the role of fractalkine in regulating cellular apoptosis/proliferation, we investigated fractalkine effects on apoptosis/proliferation signaling of granulosa cells in polycystic ovarian syndrome (PCOS) patients through in vitro and in vivo experiments. In vivo, granulosa cells were collected from 40 women undergoing oocyte retrieval (20 controls and 20 PCOS). The expression levels of fractalkine, BAX, Bcl2, Bcl2-XL, Bad, and TNF-α were assessed using RT-PCR. In vitro, we determined the effect of different doses of fractalkine on the expression of the above mentioned genes in GCs of both groups. We found that the expression levels of fractalkine and Bcl-2 were significantly lower in the GCs of PCOS patients compared to the control group (p < 0.05). In contrast, the expression levels of TNF-α and BAX were higher in the patient's group than in the control group. The results suggested that expression levels of fractalkine were negatively and positively correlated with the number of oocytes and fertilized oocytes respectively. Moreover, fractalkine could dose-dependently increase fractalkine and decrease BAD, BAX, Bcl-xl, and TNF-α expressions in the control GCs. In contrast, GCs collected from PCOS patients revealed an increase in expression of BAD, BAX, and Bcl-xl following fractalkine treatment. Our findings indicated that insufficient expression of fractalkine in PCOS patients is related with elevated apoptotic and inflammatory markers and reduced anti-apoptotic genes in the GCs.
AB - Owing to the role of fractalkine in regulating cellular apoptosis/proliferation, we investigated fractalkine effects on apoptosis/proliferation signaling of granulosa cells in polycystic ovarian syndrome (PCOS) patients through in vitro and in vivo experiments. In vivo, granulosa cells were collected from 40 women undergoing oocyte retrieval (20 controls and 20 PCOS). The expression levels of fractalkine, BAX, Bcl2, Bcl2-XL, Bad, and TNF-α were assessed using RT-PCR. In vitro, we determined the effect of different doses of fractalkine on the expression of the above mentioned genes in GCs of both groups. We found that the expression levels of fractalkine and Bcl-2 were significantly lower in the GCs of PCOS patients compared to the control group (p < 0.05). In contrast, the expression levels of TNF-α and BAX were higher in the patient's group than in the control group. The results suggested that expression levels of fractalkine were negatively and positively correlated with the number of oocytes and fertilized oocytes respectively. Moreover, fractalkine could dose-dependently increase fractalkine and decrease BAD, BAX, Bcl-xl, and TNF-α expressions in the control GCs. In contrast, GCs collected from PCOS patients revealed an increase in expression of BAD, BAX, and Bcl-xl following fractalkine treatment. Our findings indicated that insufficient expression of fractalkine in PCOS patients is related with elevated apoptotic and inflammatory markers and reduced anti-apoptotic genes in the GCs.
UR - http://www.scopus.com/inward/record.url?scp=85084207679&partnerID=8YFLogxK
U2 - 10.1007/s11033-020-05452-0
DO - 10.1007/s11033-020-05452-0
M3 - Journal articles
C2 - 32350744
AN - SCOPUS:85084207679
SN - 0301-4851
VL - 47
SP - 3593
EP - 3603
JO - Molecular biology reports
JF - Molecular biology reports
IS - 5
ER -
