TY - JOUR
T1 - Fatty acids of type 2 diabetic serum decrease the stemness properties of human adipose-derived mesenchymal stem cells
AU - Fayyazpour, Parisa
AU - Alizadeh, Effat
AU - Hosseini, Vahid
AU - Kalantary-Charvadeh, Ashkan
AU - Niafar, Mitra
AU - Sadra, Vahideh
AU - Norouzi, Zahra
AU - Saebnazar, Aysan
AU - Mehdizadeh, Amir
AU - Darabi, Masoud
N1 - Funding Information:
This paper was part of the first author's Master of Sciences thesis under the supervision of the last author. This study was supported by Endocrine Research Center at Tabriz University of Medical Sciences, Iran (Grant No. 62981). The authors would like to acknowledge the Department of Biochemistry and Clinical Laboratories at Tabriz University of Medical Sciences for supporting this project.
Funding Information:
This paper was part of the first author's Master of Sciences thesis under the supervision of the last author. This study was supported by Endocrine Research Center at Tabriz University of Medical Sciences, Iran (Grant No. 62981). The authors would like to acknowledge the Department of Biochemistry and Clinical Laboratories at Tabriz University of Medical Sciences for supporting this project.
Publisher Copyright:
© 2022 Wiley Periodicals LLC.
PY - 2022/7
Y1 - 2022/7
N2 - In type 2 diabetes, dyslipidemia and increased serum free fatty acids (FFAs) exacerbate the development of the disease through a negative effect on insulin secretion. Adipose-derived mesenchymal stem cells (AdMSCs) play a key role in regenerative medicine, and these cells can potentially be applied as novel therapeutic resources in the treatment of diabetes. In this study, AdMSCs were treated with diabetic or nondiabetic serum FFAs isolated from women of menopausal age. Serum FFAs were analyzed using gas–liquid chromatography. The expression level of the stemness markers CD49e and CD90 and the Wnt signaling target genes Axin-2 and c-Myc were evaluated using real-time PCR. The proliferation rate and colony formation were also assessed using a BrdU assay and crystal violet staining, respectively. The level of glutathione was assessed using cell fluorescence staining. Compared to nondiabetic serum, diabetic serum contained a higher percentage of oleate (1.5-fold, p < 0.01). In comparison with nondiabetic FFAs, diabetic FFAs demonstrated decreasing effects on the expression of CD90 (−51%, p < 0.001) and c-Myc (−48%, p < 0.05), and proliferation rate (−35%, p < 0.001), colony formation capacity (−50%, p < 0.01), and GSH levels (−62%, p < 0.05). The negative effect of the FFAs of diabetic serum on the stemness characteristics may impair the regenerative capabilities of AdMSCs.
AB - In type 2 diabetes, dyslipidemia and increased serum free fatty acids (FFAs) exacerbate the development of the disease through a negative effect on insulin secretion. Adipose-derived mesenchymal stem cells (AdMSCs) play a key role in regenerative medicine, and these cells can potentially be applied as novel therapeutic resources in the treatment of diabetes. In this study, AdMSCs were treated with diabetic or nondiabetic serum FFAs isolated from women of menopausal age. Serum FFAs were analyzed using gas–liquid chromatography. The expression level of the stemness markers CD49e and CD90 and the Wnt signaling target genes Axin-2 and c-Myc were evaluated using real-time PCR. The proliferation rate and colony formation were also assessed using a BrdU assay and crystal violet staining, respectively. The level of glutathione was assessed using cell fluorescence staining. Compared to nondiabetic serum, diabetic serum contained a higher percentage of oleate (1.5-fold, p < 0.01). In comparison with nondiabetic FFAs, diabetic FFAs demonstrated decreasing effects on the expression of CD90 (−51%, p < 0.001) and c-Myc (−48%, p < 0.05), and proliferation rate (−35%, p < 0.001), colony formation capacity (−50%, p < 0.01), and GSH levels (−62%, p < 0.05). The negative effect of the FFAs of diabetic serum on the stemness characteristics may impair the regenerative capabilities of AdMSCs.
UR - http://www.scopus.com/inward/record.url?scp=85132196896&partnerID=8YFLogxK
U2 - 10.1002/jcb.30270
DO - 10.1002/jcb.30270
M3 - Journal articles
C2 - 35722966
AN - SCOPUS:85132196896
SN - 0730-2312
VL - 123
SP - 1157
EP - 1170
JO - Journal of Cellular Biochemistry
JF - Journal of Cellular Biochemistry
IS - 7
ER -
