Fast RNA-RNA annealing in vitro: Single cycle selection versus multiple cycle selection

Volker Patzel; Ralf Kronenwett; Karola Rittner; Georg Sczakiel

doi:10.1080/07328319708002938

Fast RNA-RNA annealing in vitro: Single cycle selection versus multiple cycle selection

Volker Patzel, Ralf Kronenwett, Karola Rittner, Georg Sczakiel^*

^*Corresponding author for this work

Institute of Molecular Medicine

German Cancer Research Center

Abstract

Fast annealing of complementary RNA in vitro is related to effective antisense RNA-mediated regulation of gene expression and inhibit02ion of vital replication in living cells. Pools of antisense RNA can be selected for species that anneal fast with a given target strand. In this study, we compare the technical and biological advantages and disadvantages of a single round selection assay with extensive multiple cycle selection for fast- annealing antisense RNA species.

Original language	English
Journal	Nucleosides and Nucleotides
Volume	16
Issue number	5-6
Pages (from-to)	711-715
Number of pages	5
ISSN	0732-8311
DOIs	https://doi.org/10.1080/07328319708002938
Publication status	Published - 1997

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10.1080/07328319708002938

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title = "Fast RNA-RNA annealing in vitro: Single cycle selection versus multiple cycle selection",

abstract = "Fast annealing of complementary RNA in vitro is related to effective antisense RNA-mediated regulation of gene expression and inhibit02ion of vital replication in living cells. Pools of antisense RNA can be selected for species that anneal fast with a given target strand. In this study, we compare the technical and biological advantages and disadvantages of a single round selection assay with extensive multiple cycle selection for fast- annealing antisense RNA species.",

author = "Volker Patzel and Ralf Kronenwett and Karola Rittner and Georg Sczakiel",

year = "1997",

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T1 - Fast RNA-RNA annealing in vitro: Single cycle selection versus multiple cycle selection

AU - Patzel, Volker

AU - Kronenwett, Ralf

AU - Rittner, Karola

AU - Sczakiel, Georg

PY - 1997

Y1 - 1997

N2 - Fast annealing of complementary RNA in vitro is related to effective antisense RNA-mediated regulation of gene expression and inhibit02ion of vital replication in living cells. Pools of antisense RNA can be selected for species that anneal fast with a given target strand. In this study, we compare the technical and biological advantages and disadvantages of a single round selection assay with extensive multiple cycle selection for fast- annealing antisense RNA species.

AB - Fast annealing of complementary RNA in vitro is related to effective antisense RNA-mediated regulation of gene expression and inhibit02ion of vital replication in living cells. Pools of antisense RNA can be selected for species that anneal fast with a given target strand. In this study, we compare the technical and biological advantages and disadvantages of a single round selection assay with extensive multiple cycle selection for fast- annealing antisense RNA species.

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U2 - 10.1080/07328319708002938

DO - 10.1080/07328319708002938

M3 - Journal articles

AN - SCOPUS:0030755789

SN - 0732-8311

VL - 16

SP - 711

EP - 715

JO - Nucleosides and Nucleotides

JF - Nucleosides and Nucleotides

IS - 5-6

ER -

Fast RNA-RNA annealing in vitro: Single cycle selection versus multiple cycle selection

Abstract

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