Expression, purification, crystallization and preliminary X-ray diffraction analysis of chloride intracellular channel 2 (CLIC2)

Brett A. Cromer; Michael A. Gorman; Guido Hansen; Julian J. Adams; Marjorie Coggan; Philip G. Board; Michael W. Parker

doi:10.1107/S1744309107049159

Expression, purification, crystallization and preliminary X-ray diffraction analysis of chloride intracellular channel 2 (CLIC2)

Brett A. Cromer, Michael A. Gorman, Guido Hansen, Julian J. Adams, Marjorie Coggan, Philip G. Board, Michael W. Parker^*

^*Corresponding author for this work

Institute of Biochemistry

6 Citations (Scopus)

Abstract

The chloride intracellular channel (CLIC) family of proteins are unusual in that they can exist in either an integral membrane-channel form or a soluble form. Here, the expression, purification, crystallization and preliminary diffraction analysis of CLIC2, one of the least-studied members of this family, are reported. Human CLIC2 was crystallized in two different forms, both in the presence of reduced glutathione and both of which diffracted to better than 1.9 Å resolution. Crystal form A displayed P2₁2₁2 ₁ symmetry, with unit-cell parameters a = 44.0, b = 74.7, c = 79.8 Å. Crystal form B displayed P2₁ symmetry, with unit-cell parameters a = 36.0, b = 66.9, c = 44.1 Å. Structure determination will shed more light on the structure and function of this enigmatic family of proteins.

Original language	English
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	63
Issue number	11
Pages (from-to)	961-963
Number of pages	3
ISSN	1744-3091
DOIs	https://doi.org/10.1107/S1744309107049159
Publication status	Published - 2007

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Cromer, B. A., Gorman, M. A., Hansen, G., Adams, J. J., Coggan, M., Board, P. G., & Parker, M. W. (2007). Expression, purification, crystallization and preliminary X-ray diffraction analysis of chloride intracellular channel 2 (CLIC2). Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 63(11), 961-963. https://doi.org/10.1107/S1744309107049159

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title = "Expression, purification, crystallization and preliminary X-ray diffraction analysis of chloride intracellular channel 2 (CLIC2)",

abstract = "The chloride intracellular channel (CLIC) family of proteins are unusual in that they can exist in either an integral membrane-channel form or a soluble form. Here, the expression, purification, crystallization and preliminary diffraction analysis of CLIC2, one of the least-studied members of this family, are reported. Human CLIC2 was crystallized in two different forms, both in the presence of reduced glutathione and both of which diffracted to better than 1.9 {\AA} resolution. Crystal form A displayed P21212 1 symmetry, with unit-cell parameters a = 44.0, b = 74.7, c = 79.8 {\AA}. Crystal form B displayed P21 symmetry, with unit-cell parameters a = 36.0, b = 66.9, c = 44.1 {\AA}. Structure determination will shed more light on the structure and function of this enigmatic family of proteins.",

author = "Cromer, {Brett A.} and Gorman, {Michael A.} and Guido Hansen and Adams, {Julian J.} and Marjorie Coggan and Board, {Philip G.} and Parker, {Michael W.}",

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doi = "10.1107/S1744309107049159",

language = "English",

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pages = "961--963",

journal = "Acta Crystallographica Section F: Structural Biology and Crystallization Communications",

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Cromer, BA, Gorman, MA, Hansen, G, Adams, JJ, Coggan, M, Board, PG & Parker, MW 2007, 'Expression, purification, crystallization and preliminary X-ray diffraction analysis of chloride intracellular channel 2 (CLIC2)', Acta Crystallographica Section F: Structural Biology and Crystallization Communications, vol. 63, no. 11, pp. 961-963. https://doi.org/10.1107/S1744309107049159

Expression, purification, crystallization and preliminary X-ray diffraction analysis of chloride intracellular channel 2 (CLIC2). / Cromer, Brett A.; Gorman, Michael A.; Hansen, Guido et al.
In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 63, No. 11, 2007, p. 961-963.

Research output: Journal Articles › Journal articles › Research › peer-review

TY - JOUR

T1 - Expression, purification, crystallization and preliminary X-ray diffraction analysis of chloride intracellular channel 2 (CLIC2)

AU - Cromer, Brett A.

AU - Gorman, Michael A.

AU - Hansen, Guido

AU - Adams, Julian J.

AU - Coggan, Marjorie

AU - Board, Philip G.

AU - Parker, Michael W.

PY - 2007

Y1 - 2007

N2 - The chloride intracellular channel (CLIC) family of proteins are unusual in that they can exist in either an integral membrane-channel form or a soluble form. Here, the expression, purification, crystallization and preliminary diffraction analysis of CLIC2, one of the least-studied members of this family, are reported. Human CLIC2 was crystallized in two different forms, both in the presence of reduced glutathione and both of which diffracted to better than 1.9 Å resolution. Crystal form A displayed P21212 1 symmetry, with unit-cell parameters a = 44.0, b = 74.7, c = 79.8 Å. Crystal form B displayed P21 symmetry, with unit-cell parameters a = 36.0, b = 66.9, c = 44.1 Å. Structure determination will shed more light on the structure and function of this enigmatic family of proteins.

AB - The chloride intracellular channel (CLIC) family of proteins are unusual in that they can exist in either an integral membrane-channel form or a soluble form. Here, the expression, purification, crystallization and preliminary diffraction analysis of CLIC2, one of the least-studied members of this family, are reported. Human CLIC2 was crystallized in two different forms, both in the presence of reduced glutathione and both of which diffracted to better than 1.9 Å resolution. Crystal form A displayed P21212 1 symmetry, with unit-cell parameters a = 44.0, b = 74.7, c = 79.8 Å. Crystal form B displayed P21 symmetry, with unit-cell parameters a = 36.0, b = 66.9, c = 44.1 Å. Structure determination will shed more light on the structure and function of this enigmatic family of proteins.

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DO - 10.1107/S1744309107049159

M3 - Journal articles

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AN - SCOPUS:36248961582

SN - 1744-3091

VL - 63

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JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

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ER -

Expression, purification, crystallization and preliminary X-ray diffraction analysis of chloride intracellular channel 2 (CLIC2)

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