Expression changes of CAV1 and EZH2, located on 7q31∼q36, are rarely related to genomic alterations in primary prostate carcinoma

Natascha Bachmann, Juergen Haeusler, Manuel Luedeke, Rainer Kuefer, Sven Perner, Guenter Assum, Thomas Paiss, Josef Hoegel, Walther Vogel, Christiane Maier*

*Corresponding author for this work
19 Citations (Scopus)

Abstract

The chromosomal region 7q was repeatedly found to be rearranged in prostate carcinoma. It harbors several well described candidate tumor suppressor and oncogenes. We addressed two genes with opposite roles in cancer; CAV1, a putative tumor suppressor gene at 7q31, and EZH2 at 7q36, which is believed to promote tumor progression. Our primary aim was to assess their expression changes in primary tumors, and then to elucidate the underlying mechanism, assuming that genomic alterations of either locus could affect the other gene as well. In 35 prostate tumor samples, compared with adjacent tissues, CAV1 was overall downregulated (P < 10-06), whereas EZH2 was significantly overexpressed (P < 10-06). The observed dysregulations were coincident in nearly 70% of the cases. Copy number changes occurred in few tumors. Loss of CAV1 DNA was only marginally associated with reduced expression (P = 0.07), however, and genomic amplification of EZH2 could not explain its upregulation. Through bisulfite sequencing of four tumor samples, CpG-hypermethylation was verified as an alternative mechanism for CAV1 silencing, as reported previously. Moreover, it could also be involved in the reactivation of EZH2.

Original languageEnglish
JournalCancer Genetics and Cytogenetics
Volume182
Issue number2
Pages (from-to)103-110
Number of pages8
ISSN0165-4608
DOIs
Publication statusPublished - 15.04.2008

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