Exon 11 skipping of SCN10A coding for voltage-gated sodium channels in dorsal root ganglia

Jana Schirmeyer, Karol Szafranski, Enrico Leipold, Christian Mawrin, Matthias Platzer, Stefan H Heinemann

Abstract

The voltage-gated sodium channel Na(V)1.8 (encoded by SCN10A) is predominantly expressed in dorsal root ganglia(DRG) and plays a critical role in pain perception. We analyzed SCN10A transcripts isolated from human DRGs using deep sequencing and found a novel splice variant lacking exon 11, which codes for 98 amino acids of the domain I/II linker. Quantitative PCR analysis revealed an abundance of this variant of up to 5–10% in human, while no such variants were detected in mouse or rat. Since no obvious functional differences between channels with and without the exon-11 sequence were detected, it is suggested that SCN10A exon 11 skipping in humans is a tolerated event.

Original languageEnglish
JournalChannels
Volume8
Issue number3
Pages (from-to)210-5
Number of pages6
ISSN1933-6950
Publication statusPublished - 2014

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