Ex vivo and in vivo imaging of human brain tissue with different OCT systems

P. Strenge; B. Lange; C. Grill; W. Draxinger; V. Danicke; D. Theisen-Kunde; M. Bonsanto; R. Huber; R. Brinkmann

doi:10.1117/12.2526932

Ex vivo and in vivo imaging of human brain tissue with different OCT systems

P. Strenge^*, B. Lange, C. Grill, W. Draxinger, V. Danicke, D. Theisen-Kunde, M. Bonsanto, R. Huber, R. Brinkmann

^*Corresponding author for this work

Abstract

Optical coherence tomography (OCT) is a non-invasive imaging technique which is currently investigated for intraoperative detection of residual tumor during resection of human gliomas. Three different OCT systems were used for imaging of human glioblastoma in vivo (830nm spectral domain (SD) OCT integrated into a surgical microscope) and ex vivo (940nm SD-OCT and 1310nm swept-source MHz-OCT using a Fourier domain mode locked (FDML) laser). Before clinical data acquisition, the systems were characterized using a three-dimensional point-spread function phantom. To distinguish tumor from healthy brain tissue later on, attenuation coefficients of each pixel in OCT depth profiles are calculated. First examples from a clinical study show that the pixel-resolved calculation of the attenuation coefficient provides a good image contrast and confirm that white matter shows a higher signal and more homogeneous signal structure than tumorous tissue.

Original language	English
Title of host publication	Optical Coherence Imaging Techniques and Imaging in Scattering Media III
Editors	Maciej Wojtkowski, Stephen A. Boppart, Wang-Yuhl Oh
Number of pages	7
Publisher	SPIE
Publication date	19.07.2019
ISBN (Print)	978-155752820-9
DOIs	https://doi.org/10.1117/12.2526932
Publication status	Published - 19.07.2019
Event	European Conference on Biomedical Optics - Munich, Germany Duration: 23.06.2019 → 25.06.2019 Conference number: 142118

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Academic Focus: Biomedical Engineering

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10.1117/12.2526932

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Strenge, P., Lange, B., Grill, C., Draxinger, W., Danicke, V., Theisen-Kunde, D., Bonsanto, M., Huber, R., & Brinkmann, R. (2019). Ex vivo and in vivo imaging of human brain tissue with different OCT systems. In M. Wojtkowski, S. A. Boppart, & W-Y. Oh (Eds.), Optical Coherence Imaging Techniques and Imaging in Scattering Media III (Optics InfoBase Conference Papers; Vol. 110781C). SPIE. https://doi.org/10.1117/12.2526932

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title = "Ex vivo and in vivo imaging of human brain tissue with different OCT systems",

abstract = "Optical coherence tomography (OCT) is a non-invasive imaging technique which is currently investigated for intraoperative detection of residual tumor during resection of human gliomas. Three different OCT systems were used for imaging of human glioblastoma in vivo (830nm spectral domain (SD) OCT integrated into a surgical microscope) and ex vivo (940nm SD-OCT and 1310nm swept-source MHz-OCT using a Fourier domain mode locked (FDML) laser). Before clinical data acquisition, the systems were characterized using a three-dimensional point-spread function phantom. To distinguish tumor from healthy brain tissue later on, attenuation coefficients of each pixel in OCT depth profiles are calculated. First examples from a clinical study show that the pixel-resolved calculation of the attenuation coefficient provides a good image contrast and confirm that white matter shows a higher signal and more homogeneous signal structure than tumorous tissue.",

author = "P. Strenge and B. Lange and C. Grill and W. Draxinger and V. Danicke and D. Theisen-Kunde and M. Bonsanto and R. Huber and R. Brinkmann",

note = "Funding Information: This research is funded by the Federal Ministry of Education and Research Grants No.: 13GW0227A, 13GW0227B 13GW0227C. Evaluation of human brain tissue was approved by Ethics committee of University Medical Center Schleswig Holstein, Campus L{\"u}beck, Germany, No.: 18-204. We would like to thank Prof. Dr. Christian Hagel (University Hospital Eppendorf, Hamburg, Institute of histological sections. Publisher Copyright: {\textcopyright} SPIE-OSA 2019. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.; European Conference on Biomedical Optics , ECBO_2019 ; Conference date: 23-06-2019 Through 25-06-2019",

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booktitle = "Optical Coherence Imaging Techniques and Imaging in Scattering Media III",

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Strenge, P , Lange, B , Grill, C , Draxinger, W , Danicke, V , Theisen-Kunde, D , Bonsanto, M , Huber, R & Brinkmann, R 2019, Ex vivo and in vivo imaging of human brain tissue with different OCT systems. in M Wojtkowski, S A. Boppart & W-Y Oh (eds), Optical Coherence Imaging Techniques and Imaging in Scattering Media III. Optics InfoBase Conference Papers, vol. 110781C, SPIE, European Conference on Biomedical Optics , Munich, Bavaria, Germany, 23.06.19. https://doi.org/10.1117/12.2526932

Ex vivo and in vivo imaging of human brain tissue with different OCT systems. / Strenge, P.; Lange, B.; Grill, C. et al.
Optical Coherence Imaging Techniques and Imaging in Scattering Media III. ed. / Maciej Wojtkowski; Stephen A. Boppart; Wang-Yuhl Oh. SPIE, 2019. (Optics InfoBase Conference Papers; Vol. 110781C).

Research output: Chapters in Books/Reports/Conference Proceedings › Conference contribution › peer-review

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T1 - Ex vivo and in vivo imaging of human brain tissue with different OCT systems

AU - Strenge, P.

AU - Lange, B.

AU - Grill, C.

AU - Draxinger, W.

AU - Danicke, V.

AU - Theisen-Kunde, D.

AU - Bonsanto, M.

AU - Huber, R.

AU - Brinkmann, R.

N1 - Conference code: 142118

PY - 2019/7/19

Y1 - 2019/7/19

N2 - Optical coherence tomography (OCT) is a non-invasive imaging technique which is currently investigated for intraoperative detection of residual tumor during resection of human gliomas. Three different OCT systems were used for imaging of human glioblastoma in vivo (830nm spectral domain (SD) OCT integrated into a surgical microscope) and ex vivo (940nm SD-OCT and 1310nm swept-source MHz-OCT using a Fourier domain mode locked (FDML) laser). Before clinical data acquisition, the systems were characterized using a three-dimensional point-spread function phantom. To distinguish tumor from healthy brain tissue later on, attenuation coefficients of each pixel in OCT depth profiles are calculated. First examples from a clinical study show that the pixel-resolved calculation of the attenuation coefficient provides a good image contrast and confirm that white matter shows a higher signal and more homogeneous signal structure than tumorous tissue.

AB - Optical coherence tomography (OCT) is a non-invasive imaging technique which is currently investigated for intraoperative detection of residual tumor during resection of human gliomas. Three different OCT systems were used for imaging of human glioblastoma in vivo (830nm spectral domain (SD) OCT integrated into a surgical microscope) and ex vivo (940nm SD-OCT and 1310nm swept-source MHz-OCT using a Fourier domain mode locked (FDML) laser). Before clinical data acquisition, the systems were characterized using a three-dimensional point-spread function phantom. To distinguish tumor from healthy brain tissue later on, attenuation coefficients of each pixel in OCT depth profiles are calculated. First examples from a clinical study show that the pixel-resolved calculation of the attenuation coefficient provides a good image contrast and confirm that white matter shows a higher signal and more homogeneous signal structure than tumorous tissue.

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U2 - 10.1117/12.2526932

DO - 10.1117/12.2526932

M3 - Conference contribution

SN - 978-155752820-9

T3 - Optics InfoBase Conference Papers

BT - Optical Coherence Imaging Techniques and Imaging in Scattering Media III

A2 - Wojtkowski, Maciej

A2 - A. Boppart, Stephen

A2 - Oh, Wang-Yuhl

PB - SPIE

T2 - European Conference on Biomedical Optics

Y2 - 23 June 2019 through 25 June 2019

ER -

Ex vivo and in vivo imaging of human brain tissue with different OCT systems

Abstract

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