Evidence for a two proton dependent redox equilibrium in an archaeal Rieske iron-sulfur cluster

Stefan Anemüller; Christian L. Schmidt; Günter Schäfer; Eckart Bill; Alfred X. Trautwein; Miguel Teixeira

doi:10.1006/bbrc.1994.1920

Evidence for a two proton dependent redox equilibrium in an archaeal Rieske iron-sulfur cluster

Stefan Anemüller^*, Christian L. Schmidt, Günter Schäfer, Eckart Bill, Alfred X. Trautwein, Miguel Teixeira

^*Corresponding author for this work

Instituto de Tecnologia Quimica e Biologica - Univesidade Nova de Lisboa

30 Citations (Scopus)

Abstract

The recently detected Rieske iron - sulfur center in the membrane of the thermoacidophilic archaeon Sulfolobus acidocaldarius (Anemuller et al., 1993, FEBS Lett. 318, 61 - 64) was further characterized by EPR spectroscopy, coupled to redox - potentiometry and functional studies. The reduction potential is pH - dependent above pH 6, revealing the influence of two ionization equilibria in the oxidized form, with pK_a ^ox - values of 6.2 and 8.5. Above pH 9, the slope of the curve is - 120 mV/pH - unit. A partially purified fraction exerted a ubiquinol - cytochrome c oxidoreductase activity. To our knowledge, for the first time, in a membrane bound Rieske iron - sulfur protein, unequivocal evidence for a two proton dependent redox equilibrium is presented.

Original language	English
Journal	Biochemical and Biophysical Research Communications
Volume	202
Issue number	1
Pages (from-to)	252-257
Number of pages	6
ISSN	0006-291X
DOIs	https://doi.org/10.1006/bbrc.1994.1920
Publication status	Published - 15.07.1994

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10.1006/bbrc.1994.1920

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title = "Evidence for a two proton dependent redox equilibrium in an archaeal Rieske iron-sulfur cluster",

abstract = "The recently detected Rieske iron - sulfur center in the membrane of the thermoacidophilic archaeon Sulfolobus acidocaldarius (Anemuller et al., 1993, FEBS Lett. 318, 61 - 64) was further characterized by EPR spectroscopy, coupled to redox - potentiometry and functional studies. The reduction potential is pH - dependent above pH 6, revealing the influence of two ionization equilibria in the oxidized form, with pKa ox - values of 6.2 and 8.5. Above pH 9, the slope of the curve is - 120 mV/pH - unit. A partially purified fraction exerted a ubiquinol - cytochrome c oxidoreductase activity. To our knowledge, for the first time, in a membrane bound Rieske iron - sulfur protein, unequivocal evidence for a two proton dependent redox equilibrium is presented.",

author = "Stefan Anem{\"u}ller and Schmidt, \{Christian L.\} and G{\"u}nter Sch{\"a}fer and Eckart Bill and Trautwein, \{Alfred X.\} and Miguel Teixeira",

year = "1994",

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doi = "10.1006/bbrc.1994.1920",

language = "English",

volume = "202",

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journal = "Biochemical and Biophysical Research Communications",

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T1 - Evidence for a two proton dependent redox equilibrium in an archaeal Rieske iron-sulfur cluster

AU - Anemüller, Stefan

AU - Schmidt, Christian L.

AU - Schäfer, Günter

AU - Bill, Eckart

AU - Trautwein, Alfred X.

AU - Teixeira, Miguel

PY - 1994/7/15

Y1 - 1994/7/15

N2 - The recently detected Rieske iron - sulfur center in the membrane of the thermoacidophilic archaeon Sulfolobus acidocaldarius (Anemuller et al., 1993, FEBS Lett. 318, 61 - 64) was further characterized by EPR spectroscopy, coupled to redox - potentiometry and functional studies. The reduction potential is pH - dependent above pH 6, revealing the influence of two ionization equilibria in the oxidized form, with pKa ox - values of 6.2 and 8.5. Above pH 9, the slope of the curve is - 120 mV/pH - unit. A partially purified fraction exerted a ubiquinol - cytochrome c oxidoreductase activity. To our knowledge, for the first time, in a membrane bound Rieske iron - sulfur protein, unequivocal evidence for a two proton dependent redox equilibrium is presented.

AB - The recently detected Rieske iron - sulfur center in the membrane of the thermoacidophilic archaeon Sulfolobus acidocaldarius (Anemuller et al., 1993, FEBS Lett. 318, 61 - 64) was further characterized by EPR spectroscopy, coupled to redox - potentiometry and functional studies. The reduction potential is pH - dependent above pH 6, revealing the influence of two ionization equilibria in the oxidized form, with pKa ox - values of 6.2 and 8.5. Above pH 9, the slope of the curve is - 120 mV/pH - unit. A partially purified fraction exerted a ubiquinol - cytochrome c oxidoreductase activity. To our knowledge, for the first time, in a membrane bound Rieske iron - sulfur protein, unequivocal evidence for a two proton dependent redox equilibrium is presented.

UR - https://www.scopus.com/pages/publications/0028026972

U2 - 10.1006/bbrc.1994.1920

DO - 10.1006/bbrc.1994.1920

M3 - Journal articles

AN - SCOPUS:0028026972

SN - 0006-291X

VL - 202

SP - 252

EP - 257

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Evidence for a two proton dependent redox equilibrium in an archaeal Rieske iron-sulfur cluster

Abstract

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