TY - JOUR
T1 - Evaluation of a RT-PCR based routine screening tool for the detection of disseminated epithelial cells in the bone marrow of breast cancer patients
AU - Becker, Sven
AU - Becker-Pergola, Graziella
AU - Banys, Malgorzata
AU - Krawczyk, Natalia
AU - Wallwiener, Diethelm
AU - Solomayer, Erich
AU - Schuetz, Christina
AU - Fehm, Tanja
N1 - Funding Information:
Acknowledgements This work was supported by the University of Tübingen AKF Foundation (Angewandte Klinische Forschung).
PY - 2009/9
Y1 - 2009/9
N2 - Purpose: Numerous studies have shown that the presence of clinically occult disseminated tumor cells (DTC's) in the bone marrow (BM) of breast cancer patients is associated with an unfavourable clinical outcome. Immunocytochemistry (ICC) remains the gold standard for their detection. While assays based on RT-PCR are available, they have not been used for routine detection of DTC's. Methods: To assess the quality of the assay, we performed a direct comparison of DTC detection rates in a large cohort of 385 patients using both standardized ICC and real-time RT-PCR protocols. Correlation rates were assessed, and results were compared with clinical data. Results: A significant correlation between ICC and RT-PCR was observed (P < 0.01). Positivity rates were similar (both 35%) and the results of both methods agreed in 73% of cases (280/385). Conclusions: We describe a real-time RT-PCR based protocol for DTC-detection that has been specifically designed for routine clinical laboratory use. As such, RT-PCR has the potential to become an alternative testing method for BM evaluation in breast cancer patients.
AB - Purpose: Numerous studies have shown that the presence of clinically occult disseminated tumor cells (DTC's) in the bone marrow (BM) of breast cancer patients is associated with an unfavourable clinical outcome. Immunocytochemistry (ICC) remains the gold standard for their detection. While assays based on RT-PCR are available, they have not been used for routine detection of DTC's. Methods: To assess the quality of the assay, we performed a direct comparison of DTC detection rates in a large cohort of 385 patients using both standardized ICC and real-time RT-PCR protocols. Correlation rates were assessed, and results were compared with clinical data. Results: A significant correlation between ICC and RT-PCR was observed (P < 0.01). Positivity rates were similar (both 35%) and the results of both methods agreed in 73% of cases (280/385). Conclusions: We describe a real-time RT-PCR based protocol for DTC-detection that has been specifically designed for routine clinical laboratory use. As such, RT-PCR has the potential to become an alternative testing method for BM evaluation in breast cancer patients.
UR - http://www.scopus.com/inward/record.url?scp=68949092088&partnerID=8YFLogxK
U2 - 10.1007/s10549-008-0174-3
DO - 10.1007/s10549-008-0174-3
M3 - Journal articles
C2 - 18791864
AN - SCOPUS:68949092088
SN - 0167-6806
VL - 117
SP - 227
EP - 233
JO - Breast Cancer Research and Treatment
JF - Breast Cancer Research and Treatment
IS - 2
ER -
