TY - JOUR
T1 - Etoposide Upregulates Survival Favoring Sphingosine-1-Phosphate in Etoposide-Resistant Retinoblastoma Cells
AU - Kakkassery, Vinodh
AU - Skosyrski, S.
AU - Lüth, A.
AU - Kleuser, B.
AU - van der Giet, M.
AU - Tate, R.
AU - Reinhard, J.
AU - Faissner, A.
AU - Joachim, S. C.
AU - Kociok, N.
PY - 2019/1/15
Y1 - 2019/1/15
N2 - Improved knowledge of retinoblastoma chemotherapy resistance is needed to raise treatment efficiency. The objective of this study was to test whether etoposide alters glucosyl-ceramide, ceramide, sphingosine, and sphingosine-1-phosphate (sphingosine-1-P) levels in parental retinoblastoma cells (WERI Rb1) or their etoposide-resistant subclones (WERI EtoR). WERI Rb1 and WERI EtoR were incubated with 400 ng/ml etoposide for 24 h. Levels of glucosyl-ceramides, ceramides, sphingosine, sphingosine-1-P were detected by Q-TOF mass spectrometry. Statistical analysis was done by ANOVA followed by Tukey post-hoc test (p 0.2). Both cell lines upregulate pro-apoptotic sphingosine after etoposide incubation, but only WERI EtoR produces additional survival favorable sphingosine-1-P. These data may suggest a role of sphingosine-1-P in retinoblastoma chemotherapy resistance, although this seems not to be the only resistance mechanism.
AB - Improved knowledge of retinoblastoma chemotherapy resistance is needed to raise treatment efficiency. The objective of this study was to test whether etoposide alters glucosyl-ceramide, ceramide, sphingosine, and sphingosine-1-phosphate (sphingosine-1-P) levels in parental retinoblastoma cells (WERI Rb1) or their etoposide-resistant subclones (WERI EtoR). WERI Rb1 and WERI EtoR were incubated with 400 ng/ml etoposide for 24 h. Levels of glucosyl-ceramides, ceramides, sphingosine, sphingosine-1-P were detected by Q-TOF mass spectrometry. Statistical analysis was done by ANOVA followed by Tukey post-hoc test (p 0.2). Both cell lines upregulate pro-apoptotic sphingosine after etoposide incubation, but only WERI EtoR produces additional survival favorable sphingosine-1-P. These data may suggest a role of sphingosine-1-P in retinoblastoma chemotherapy resistance, although this seems not to be the only resistance mechanism.
UR - http://www.scopus.com/inward/record.url?scp=85034083044&partnerID=8YFLogxK
UR - http://www.mendeley.com/research/etoposide-upregulates-survival-favoring-sphingosine1phosphate-etoposideresistant-retinoblastoma-cell
U2 - 10.1007/s12253-017-0360-x
DO - 10.1007/s12253-017-0360-x
M3 - Journal articles
C2 - 29143233
AN - SCOPUS:85034083044
SN - 1219-4956
VL - 25
SP - 391
EP - 399
JO - Pathology and Oncology Research
JF - Pathology and Oncology Research
IS - 1
ER -
