Estrogenic and antiestrogenic regulation of MMP-2 and MMP-13 mRNA in RUCA-I endometrial tumor cells in vitro and in vivo

Ludger Tüshaus, Anne Christine Hopert*, Elisabeth Strunck, Christine Schubert, Winfried Wünsche, G. ünter Vollmer

*Corresponding author for this work
10 Citations (Scopus)

Abstract

We investigated the influence of estrogenic and antiestrogenic treatment on proteolytic activity - especially on MMP-2 and MMP-13 - in the RUCA-I transplantable endometrial tumor model. Morphological studies demonstrate that RUCA-I cells are forming highly differentiated gland-like structures by remodelling and invading the underlying ECM. Estrogens upregulate the mRNA levels of MMP-2 and MMP-13 in the rat uterus. Treatment with the pure antiestrogen ICI 182,780 results in the downregulation of MMP-2 and MMP-13 mRNA. The same regulation for MMP-13 mRNA is found in vitro in RUCA-I cells. In contrast, in the transplantation tumor, the mRNA level of MMP-13 is repressed by estrogens and induced by ICI 182,780. MMP-2 mRNA is not regulated by hormones in the transplantation tumor and in RUCA-I cells. The divergent regulation suggests a varying influence of cell-cell-, cell-extracellular matrix interactions and soluble factors.

Original languageEnglish
JournalCancer Letters
Volume198
Issue number1
Pages (from-to)99-106
Number of pages8
ISSN0304-3835
DOIs
Publication statusPublished - 30.07.2003

Research Areas and Centers

  • Academic Focus: Center for Brain, Behavior and Metabolism (CBBM)

Fingerprint

Dive into the research topics of 'Estrogenic and antiestrogenic regulation of MMP-2 and MMP-13 mRNA in RUCA-I endometrial tumor cells in vitro and in vivo'. Together they form a unique fingerprint.

Cite this