Abstract
We investigated the influence of estrogenic and antiestrogenic treatment on proteolytic activity - especially on MMP-2 and MMP-13 - in the RUCA-I transplantable endometrial tumor model. Morphological studies demonstrate that RUCA-I cells are forming highly differentiated gland-like structures by remodelling and invading the underlying ECM. Estrogens upregulate the mRNA levels of MMP-2 and MMP-13 in the rat uterus. Treatment with the pure antiestrogen ICI 182,780 results in the downregulation of MMP-2 and MMP-13 mRNA. The same regulation for MMP-13 mRNA is found in vitro in RUCA-I cells. In contrast, in the transplantation tumor, the mRNA level of MMP-13 is repressed by estrogens and induced by ICI 182,780. MMP-2 mRNA is not regulated by hormones in the transplantation tumor and in RUCA-I cells. The divergent regulation suggests a varying influence of cell-cell-, cell-extracellular matrix interactions and soluble factors.
Original language | English |
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Journal | Cancer Letters |
Volume | 198 |
Issue number | 1 |
Pages (from-to) | 99-106 |
Number of pages | 8 |
ISSN | 0304-3835 |
DOIs | |
Publication status | Published - 30.07.2003 |
Research Areas and Centers
- Academic Focus: Center for Brain, Behavior and Metabolism (CBBM)