Enrichment and detection of live antigen-specific CD4+ and CD8+ T cells based on cytokine secretion

Helmut Brosterhus; Susanne Brings; Heike Leyendeckers; Rudolf A. Manz; Stefan Miltenyi; Andreas Radbruch; Mario Assenmacher; Jürgen Schmitz

doi:10.1002/(SICI)1521-4141(199912)29:12<4053::AID-IMMU4053>3.0.CO;2-C

Enrichment and detection of live antigen-specific CD4⁺ and CD8⁺ T cells based on cytokine secretion

Helmut Brosterhus, Susanne Brings, Heike Leyendeckers, Rudolf A. Manz, Stefan Miltenyi, Andreas Radbruch, Mario Assenmacher, Jürgen Schmitz^*

^*Corresponding author for this work

Institute of Systemic Inflamation Research

162 Citations (Scopus)

Abstract

Following appropriate antigen-specific stimulation, CD4⁺ and CD8⁺ T lymphocytes rapidly express cytokines. Based on this stimulation-induced cytokine secretion and using cell suface affinity matrix technology we have developed a new method that permits specific, rapid and efficient detection, isolation and characterization of live antigen-specific CD4⁺ and CD8⁺ T lymphocytes. The power of this technique is demonstrated here for HL4-A0201-restricted influenza matrix protein peptide 58-66-specific CD8⁺ cytotoxic T lymphocytes, influenza A virus- and recombinant tetanus toxin C fragment-specific Th1 cells and tetanus toxoid-specific Th2 cells.

Original language	English
Journal	European Journal of Immunology
Volume	29
Issue number	12
Pages (from-to)	4053-4059
Number of pages	7
ISSN	0014-2980
DOIs	https://doi.org/10.1002/(SICI)1521-4141(199912)29:12<4053::AID-IMMU4053>3.0.CO;2-C
Publication status	Published - 1999

Research Areas and Centers

Academic Focus: Center for Infection and Inflammation Research (ZIEL)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1002/(SICI)1521-4141(199912)29:12<4053::AID-IMMU4053>3.0.CO;2-C

Cite this

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title = "Enrichment and detection of live antigen-specific CD4+ and CD8+ T cells based on cytokine secretion",

abstract = "Following appropriate antigen-specific stimulation, CD4+ and CD8+ T lymphocytes rapidly express cytokines. Based on this stimulation-induced cytokine secretion and using cell suface affinity matrix technology we have developed a new method that permits specific, rapid and efficient detection, isolation and characterization of live antigen-specific CD4+ and CD8+ T lymphocytes. The power of this technique is demonstrated here for HL4-A0201-restricted influenza matrix protein peptide 58-66-specific CD8+ cytotoxic T lymphocytes, influenza A virus- and recombinant tetanus toxin C fragment-specific Th1 cells and tetanus toxoid-specific Th2 cells.",

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T1 - Enrichment and detection of live antigen-specific CD4+ and CD8+ T cells based on cytokine secretion

AU - Brosterhus, Helmut

AU - Brings, Susanne

AU - Leyendeckers, Heike

AU - Manz, Rudolf A.

AU - Miltenyi, Stefan

AU - Radbruch, Andreas

AU - Assenmacher, Mario

AU - Schmitz, Jürgen

PY - 1999

Y1 - 1999

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AB - Following appropriate antigen-specific stimulation, CD4+ and CD8+ T lymphocytes rapidly express cytokines. Based on this stimulation-induced cytokine secretion and using cell suface affinity matrix technology we have developed a new method that permits specific, rapid and efficient detection, isolation and characterization of live antigen-specific CD4+ and CD8+ T lymphocytes. The power of this technique is demonstrated here for HL4-A0201-restricted influenza matrix protein peptide 58-66-specific CD8+ cytotoxic T lymphocytes, influenza A virus- and recombinant tetanus toxin C fragment-specific Th1 cells and tetanus toxoid-specific Th2 cells.

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