TY - JOUR
T1 - Enhanced production of pro-inflammatory cytokines and chemokines in Ethiopian cutaneous leishmaniasis upon exposure to Leishmania aethiopica
AU - Chanyalew, Menberework
AU - Abebe, Markos
AU - Endale, Birtukan
AU - Girma, Selfu
AU - Tasew, Geremew
AU - van Zandbergen, Ger
AU - Ritter, Uwe
AU - Gadisa, Endalamaw
AU - Aseffa, Abraham
AU - Laskay, Tamás
N1 - Funding Information:
This study was supported by German Research Foundation / Deutsche Forschungsgemeinschaft (DFG) grant for the German-African cooperation project “Innate immunity in leishmaniasis” ( RI 1849/2-1 , LA 1267/3-1 and ZA 533/5-1 ) and by the Armauer Hansen Research Institute (AHRI) Sida / Norad core-funding).
Publisher Copyright:
© 2020 Elsevier Ltd
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/8/18
Y1 - 2020/8/18
N2 - The clinical course and outcome of cutaneous leishmaniasis (CL) vary due to the infecting Leishmania species and host genetic makeup that result in different immune responses against the parasites. The host immune response to Leishmania aethiopica (L. aethiopica), the causative agent of CL in Ethiopia, is poorly understood. To contribute to the understanding of the protective immune response in CL due to L. aethiopica, we characterized the cytokine response to L. aethiopica in patients with the localized form of CL (LCL) and age-and sex-matched apparently healthy controls. By applying a whole blood based in vitro culture we found enhanced release of TNF, IL–6, MCP-1 or CCL2, IP-10 or CXCL10, MIP-1β or CCL4 and IL-8 or CXCL8- but not of IL–10CL patients in response to L. aethiopica compared to the controls. No difference was observed between LCL cases and controls in the secretion of these cytokines and chemokines in whole blood cultures treated with the TLR-ligands LPS, MALP-2 or poly I: C. The observed increased secretion of the pro-inflammatory cytokines/chemokines reflects an enhanced response against the parasites by LCL patients as compared to healthy controls rather than a generally enhanced ability of blood leukocytes from LCL patients to respond to microbial constituents. Our findings suggest that the enhanced production of pro-inflammatory cytokines/chemokines is associated with localized cutaneous leishmaniasis caused by L. aethiopica.
AB - The clinical course and outcome of cutaneous leishmaniasis (CL) vary due to the infecting Leishmania species and host genetic makeup that result in different immune responses against the parasites. The host immune response to Leishmania aethiopica (L. aethiopica), the causative agent of CL in Ethiopia, is poorly understood. To contribute to the understanding of the protective immune response in CL due to L. aethiopica, we characterized the cytokine response to L. aethiopica in patients with the localized form of CL (LCL) and age-and sex-matched apparently healthy controls. By applying a whole blood based in vitro culture we found enhanced release of TNF, IL–6, MCP-1 or CCL2, IP-10 or CXCL10, MIP-1β or CCL4 and IL-8 or CXCL8- but not of IL–10CL patients in response to L. aethiopica compared to the controls. No difference was observed between LCL cases and controls in the secretion of these cytokines and chemokines in whole blood cultures treated with the TLR-ligands LPS, MALP-2 or poly I: C. The observed increased secretion of the pro-inflammatory cytokines/chemokines reflects an enhanced response against the parasites by LCL patients as compared to healthy controls rather than a generally enhanced ability of blood leukocytes from LCL patients to respond to microbial constituents. Our findings suggest that the enhanced production of pro-inflammatory cytokines/chemokines is associated with localized cutaneous leishmaniasis caused by L. aethiopica.
UR - http://www.scopus.com/inward/record.url?scp=85091261226&partnerID=8YFLogxK
U2 - 10.1016/j.cyto.2020.155289
DO - 10.1016/j.cyto.2020.155289
M3 - Journal articles
AN - SCOPUS:85091261226
SN - 1043-4666
JO - Cytokine
JF - Cytokine
M1 - 155289
ER -
