TY - JOUR
T1 - Elevated interleukin-4 and interleukin-13 production by T cell lines from patients with Churg-Strauss syndrome
AU - Kiene, M.
AU - Csernok, E.
AU - Müller, A.
AU - Metzler, C.
AU - Trabandt, A.
AU - Gross, W. L.
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2001
Y1 - 2001
N2 - Objective. To investigate cytokine production patterns of T cell lines (TCL) from patients with Churg-Strauss syndrome (CSS). Methods. Short-term polyclonal TCL were generated from peripheral blood of patients with CSS or Wegener's granulomatosis (WG) and healthy controls (HC). TCL were established in the presence of interleukin-2 (IL-2) and phytohemagglutinin and were phenotypically characterized by flow cytometry. Th1/ Th2 cytokine production by stimulated TCL (72 hours) was analyzed by enzyme-linked immunosorbent assay. Results. TCL that represented the progeny of in vivo-activated T cells from CSS patients displayed a heterogeneous immunophenotype, with a predominance of CD4+ T cells when compared with WG TCL, which were predominantly CD8+. All CSS TCL shared the ability to produce large amounts of interferon-γ (IFNγ), IL-4, and IL-13 compared with HC (P = 0.014 for all 3). Production of IL-4 and IL-13 was higher in CSS TCL than in WG TCL (P = 0.014 for both). IL-5 production was up-regulated in WG TCL compared with CSS TCL (P = 0.014). Compared with HC, WG TCL showed increased production of IFNγ (P = 0.021), IL-5 (P = 0.043), and IL-13 (P = 0.021). Conclusion. Our results indicate that, while there is evidence for both a type 1 and a type 2 response in CSS, type 2 cytokine production pattern appears to predominate in this disease when compared with WG and HC.
AB - Objective. To investigate cytokine production patterns of T cell lines (TCL) from patients with Churg-Strauss syndrome (CSS). Methods. Short-term polyclonal TCL were generated from peripheral blood of patients with CSS or Wegener's granulomatosis (WG) and healthy controls (HC). TCL were established in the presence of interleukin-2 (IL-2) and phytohemagglutinin and were phenotypically characterized by flow cytometry. Th1/ Th2 cytokine production by stimulated TCL (72 hours) was analyzed by enzyme-linked immunosorbent assay. Results. TCL that represented the progeny of in vivo-activated T cells from CSS patients displayed a heterogeneous immunophenotype, with a predominance of CD4+ T cells when compared with WG TCL, which were predominantly CD8+. All CSS TCL shared the ability to produce large amounts of interferon-γ (IFNγ), IL-4, and IL-13 compared with HC (P = 0.014 for all 3). Production of IL-4 and IL-13 was higher in CSS TCL than in WG TCL (P = 0.014 for both). IL-5 production was up-regulated in WG TCL compared with CSS TCL (P = 0.014). Compared with HC, WG TCL showed increased production of IFNγ (P = 0.021), IL-5 (P = 0.043), and IL-13 (P = 0.021). Conclusion. Our results indicate that, while there is evidence for both a type 1 and a type 2 response in CSS, type 2 cytokine production pattern appears to predominate in this disease when compared with WG and HC.
UR - http://www.scopus.com/inward/record.url?scp=0035097324&partnerID=8YFLogxK
U2 - 10.1002/1529-0131(200102)44:2<469::AID-ANR66>3.0.CO;2-0
DO - 10.1002/1529-0131(200102)44:2<469::AID-ANR66>3.0.CO;2-0
M3 - Journal articles
C2 - 11229479
AN - SCOPUS:0035097324
SN - 0004-3591
VL - 44
SP - 469
EP - 473
JO - Arthritis and Rheumatism
JF - Arthritis and Rheumatism
IS - 2
ER -