Electrical high frequency stimulation of the caudate nucleus induces local GABA outflow in freely moving rats

A. Hiller; S. Loeffler; C. Haupt; M. Litza; U. Hofmann; A. Moser

doi:10.1016/j.jneumeth.2006.07.023

Electrical high frequency stimulation of the caudate nucleus induces local GABA outflow in freely moving rats

A. Hiller, S. Loeffler, C. Haupt, M. Litza, U. Hofmann, A. Moser^*

^*Corresponding author for this work

22 Citations (Scopus)

Abstract

Electrical high frequency stimulation of the globus pallidus internus or the subthalamic nucleus has beneficial motor effects in advanced Parkinson's disease. The mechanisms underlying these clinical results remain, however, unclear. From previous in vitro studies it is proposed that the γ-aminobutyric acid (GABA) system is involved in the effectiveness of electrical high frequency stimulation (HFS). In these experiments, we developed an in vivo model that allows for simultaneous and collocated microdialysis and HFS by electrical pulses of 124 Hz in the caudate nucleus of freely moving rats. GABA and glutamate outflow were sampled by microdialysis technique and quantified after pre-column o-phthaldialdehyde sulphite derivatization using HPLC with electrochemical detection. As the most outstanding result, we could demonstrate that high frequency stimulation significantly increased basal GABA outflow without affecting glutamate levels in freely moving rats.

Original language	English
Journal	Journal of Neuroscience Methods
Volume	159
Issue number	2
Pages (from-to)	286-290
Number of pages	5
ISSN	0165-0270
DOIs	https://doi.org/10.1016/j.jneumeth.2006.07.023
Publication status	Published - 30.01.2007

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10.1016/j.jneumeth.2006.07.023

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title = "Electrical high frequency stimulation of the caudate nucleus induces local GABA outflow in freely moving rats",

abstract = "Electrical high frequency stimulation of the globus pallidus internus or the subthalamic nucleus has beneficial motor effects in advanced Parkinson's disease. The mechanisms underlying these clinical results remain, however, unclear. From previous in vitro studies it is proposed that the γ-aminobutyric acid (GABA) system is involved in the effectiveness of electrical high frequency stimulation (HFS). In these experiments, we developed an in vivo model that allows for simultaneous and collocated microdialysis and HFS by electrical pulses of 124 Hz in the caudate nucleus of freely moving rats. GABA and glutamate outflow were sampled by microdialysis technique and quantified after pre-column o-phthaldialdehyde sulphite derivatization using HPLC with electrochemical detection. As the most outstanding result, we could demonstrate that high frequency stimulation significantly increased basal GABA outflow without affecting glutamate levels in freely moving rats.",

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T1 - Electrical high frequency stimulation of the caudate nucleus induces local GABA outflow in freely moving rats

AU - Hiller, A.

AU - Loeffler, S.

AU - Haupt, C.

AU - Litza, M.

AU - Hofmann, U.

AU - Moser, A.

PY - 2007/1/30

Y1 - 2007/1/30

N2 - Electrical high frequency stimulation of the globus pallidus internus or the subthalamic nucleus has beneficial motor effects in advanced Parkinson's disease. The mechanisms underlying these clinical results remain, however, unclear. From previous in vitro studies it is proposed that the γ-aminobutyric acid (GABA) system is involved in the effectiveness of electrical high frequency stimulation (HFS). In these experiments, we developed an in vivo model that allows for simultaneous and collocated microdialysis and HFS by electrical pulses of 124 Hz in the caudate nucleus of freely moving rats. GABA and glutamate outflow were sampled by microdialysis technique and quantified after pre-column o-phthaldialdehyde sulphite derivatization using HPLC with electrochemical detection. As the most outstanding result, we could demonstrate that high frequency stimulation significantly increased basal GABA outflow without affecting glutamate levels in freely moving rats.

AB - Electrical high frequency stimulation of the globus pallidus internus or the subthalamic nucleus has beneficial motor effects in advanced Parkinson's disease. The mechanisms underlying these clinical results remain, however, unclear. From previous in vitro studies it is proposed that the γ-aminobutyric acid (GABA) system is involved in the effectiveness of electrical high frequency stimulation (HFS). In these experiments, we developed an in vivo model that allows for simultaneous and collocated microdialysis and HFS by electrical pulses of 124 Hz in the caudate nucleus of freely moving rats. GABA and glutamate outflow were sampled by microdialysis technique and quantified after pre-column o-phthaldialdehyde sulphite derivatization using HPLC with electrochemical detection. As the most outstanding result, we could demonstrate that high frequency stimulation significantly increased basal GABA outflow without affecting glutamate levels in freely moving rats.

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Electrical high frequency stimulation of the caudate nucleus induces local GABA outflow in freely moving rats

Abstract

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Graduate School GSC 235: Graduate School for Computing in Medicine and Life Sciences

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Electrical high frequency stimulation of the caudate nucleus induces local GABA outflow in freely moving rats

Abstract

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Graduate School GSC 235: Graduate School for Computing in Medicine and Life Sciences

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