Einfluss der Leukozytenkontamination auf die Lagerbarkeit von Thrombozytenkonzentraten aus gepooltem Buffy coat.

Translated title of the contribution: Effect of leukocyte contamination on storage of platelet concentrates from buffy coats

H. Klüter*, M. Klinger, M. Bauhaus, H. Kirchner

*Corresponding author for this work
1 Citation (Scopus)


We examined the effect of white cell contamination on thrombocytes prepared from pooled buffy coats over a storage period of 8 days. Using this novel technique, a leukocyte depletion filter can be easily integrated during PC preparation. In a paired study (n = 14) eight ABO-identical BC were pooled in a 2-liter PVC bag within 8 h after whole-blood donation, thoroughly mixed and divided into two identical fractions. After soft-spin centrifugation the platelet-rich plasma (PRP) was transferred either (fraction A) using a leukocyte filter (PL 50-HF, Pall) or (fraction B) directly into the storage bag (Pl-732, Baxter), and stored under routine conditions. On days 1, 3, 5, and 8, aliquots of PC were withdrawn for determination of cell count and different biochemical parameters and for morphometric analyses of platelet ultrastructure by electron microscopy. Results showed a lower thrombocyte yield and white cell count (p < 0.01) in fraction A (268 x 10(9) vs. 240 x 10(9); 51.1 x 10(6) vs. 0.04 x 10(6)), whereas no differences between the preparations could be detected by analysis of pH, pCO2, bicarbonate, and in LDH release over the storage period of 8 days. These results were supported in the study on the ultrastructural level where a good morphological integrity of the platelets was observed during the whole storage period in both fractions. In conclusion, storage lesions on platelets due to leukocyte effects are unlikely to occur in PC with white cell counts lower than 10(8)/l.

Translated title of the contributionEffect of leukocyte contamination on storage of platelet concentrates from buffy coats
Original languageGerman
JournalBeiträge zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine
Pages (from-to)61-65
Number of pages5
Publication statusPublished - 1994

Research Areas and Centers

  • Academic Focus: Center for Infection and Inflammation Research (ZIEL)


Dive into the research topics of 'Effect of leukocyte contamination on storage of platelet concentrates from buffy coats'. Together they form a unique fingerprint.

Cite this