Effekt einer neuen oberflächenbehandlung von intraokularlinsen aus silikon durch ein fluoralkylsilan auf das adhärenzverhalten endophthalmitis-relevanter bakterien in vitro

Introduction. Dynasilan® is a fluoroalkylsilan which is able to bind to surface active molecules of intraocular lenses (IOLs), thereby offering a new option for surface modification of silicone lenses. The purpose of this in vitro study was to investigate the influence of this new surface treatment on the adherence of two typical endophthalmitis-inducing bacteria (Staphylococcus epidermidis, Propionibacterium acnes). Materials and methods. A total of 14 Dynasilan®-treated and 14 untreated silicone lenses were incubated at 37°C for 24 h in brain heart infusion broth (108 CFU/ml) either with Staphylococcus epidermidis or with Propionibacterium acnes for 1 h. Subsequently, the adherent bacteria were resuspended using ultrasonification at 35 kHz for 3×45 s. After a dilution series and incubation at 37°C for 24 h or 3 days the colonies were counted. Results. On untreated IOLs incubated with Staphylococcus epidermidis the average number of bacteria was 3.6×10⁷/ml, and on treated IOLs the number of counted colonies was reduced to 1.09×10⁷/ml. Incubated with Propionibacterium acnes the average number of adherent bacteria on untreated IOLs was 4.75×10⁴/ml and on modified IOLs the number was reduced to 2.94×10⁴/ml. Conclusion. Dynasilan® surface treatment may reduce the adherence of Staphylococcus epidermidis and Propionibacterium acnes on silicone intraocular lenses. Further studies regarding the stability of this treatment, its biocompatibility and influence on lens epithelial cell adhesion are in progress.