TY - JOUR
T1 - Effects of the helium-neon laser on rat kidney epithelial cells in culture
AU - Schneede, Peter
AU - Jelkmann, Wolfgang
AU - Schramm, Uda
AU - Fricke, Harald
AU - Steinmetz, Manfred
AU - Hofstetter, Alfons
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 1988/3
Y1 - 1988/3
N2 - The cellular mechanisms of action of low power lasers are only partially understood. Therefore, we have studied effects of helium-neon (He-Ne) laser irradiation in a cell culture model. The studies were performed with the permanent rat kidney cell line RK-L. By electron microscopy, significant ultrastructural changes of the cells were seen after He-Ne irradiation at 40 mW/cm2 for 4h (569 J/cm2), including detritus-like formations of the cell organelles close to the nucleus. While such changes were not observed after 1 h irradiation (142 J/cm2), the number of cells in mitosis increased under this condition. In addition, the rate of incorporation of3H-thymidine into DNA decreased transiently 6-9 h after 1 h He-Ne irradiation of the cells. The consumption of glucose was also lowered for 10 h after irradiation, while the production of lactate increased. Finally, the synthesis of prostaglandin E2 was reduced following irradiation. These results show that the He-Ne laser induces significant effects at the cellular level. In addition, our findings support the concept that the biological effects of low power laser treatment involve changes in the cell cycle.
AB - The cellular mechanisms of action of low power lasers are only partially understood. Therefore, we have studied effects of helium-neon (He-Ne) laser irradiation in a cell culture model. The studies were performed with the permanent rat kidney cell line RK-L. By electron microscopy, significant ultrastructural changes of the cells were seen after He-Ne irradiation at 40 mW/cm2 for 4h (569 J/cm2), including detritus-like formations of the cell organelles close to the nucleus. While such changes were not observed after 1 h irradiation (142 J/cm2), the number of cells in mitosis increased under this condition. In addition, the rate of incorporation of3H-thymidine into DNA decreased transiently 6-9 h after 1 h He-Ne irradiation of the cells. The consumption of glucose was also lowered for 10 h after irradiation, while the production of lactate increased. Finally, the synthesis of prostaglandin E2 was reduced following irradiation. These results show that the He-Ne laser induces significant effects at the cellular level. In addition, our findings support the concept that the biological effects of low power laser treatment involve changes in the cell cycle.
UR - http://www.scopus.com/inward/record.url?scp=52449147464&partnerID=8YFLogxK
U2 - 10.1007/BF02593820
DO - 10.1007/BF02593820
M3 - Journal articles
AN - SCOPUS:52449147464
SN - 0268-8921
VL - 3
SP - 249
EP - 257
JO - Lasers In Medical Science
JF - Lasers In Medical Science
IS - 1-4
ER -