TY - JOUR
T1 - Effects of pigment epithelium-derived factor on traumatic brain injury
AU - Yusuf Terzi, Menderes
AU - Casalis, Pablo
AU - Lang, Veronika
AU - Zille, Marietta
AU - Bründl, Elisabeth
AU - Störr, Eva Maria
AU - Brawanski, Alexander
AU - Vajkoczy, Peter
AU - Thomale, Ulrich
AU - Piña, Ana Luisa
N1 - Publisher Copyright:
© 2015 - IOS Press and the authors. All rights reserved.
PY - 2015
Y1 - 2015
N2 - Purpose: Pigment epithelium-derived factor (PEDF) is a multifunctional protein with antiangiogenic, anti-inflammatory, neurotrophic and neurogenic properties. The effect of PEDF on traumatic brain injury (TBI) has not been explored. In this study, we aimed to show the in vivo effects of PEDF on lesion volume, cell death and cell proliferation after TBI. Methods: Rats were subjected tocontrolled cortical impact injury (CCII). PEDF mRNA brain levels were measured by RT-PCR. The lesion volume, cell proliferation, cell death and microglia activation were assessed in the brains of lesioned animals with intraventricular alzet infusion of PEDF or aCSF, and intraperitoneal injections of BrdU. Results: We detected a significant increase of PEDF mRNA levels after TBI. PEDF intraventricular infusions howed no significant effect on the contusion volume, whereas the number of dead cells, activated microglia, BrdU-positive cells around the lesion were significantly decreased. In contrast, PEDF application increased cell proliferation in the ipsilateral subventricular zone. No effect was found on cell proliferation in the dentate gyrus. Conclusion: The present work indicates that PEDF acts as a multifunctional agent after TBI influencing cell death, inflammation and cell proliferation.
AB - Purpose: Pigment epithelium-derived factor (PEDF) is a multifunctional protein with antiangiogenic, anti-inflammatory, neurotrophic and neurogenic properties. The effect of PEDF on traumatic brain injury (TBI) has not been explored. In this study, we aimed to show the in vivo effects of PEDF on lesion volume, cell death and cell proliferation after TBI. Methods: Rats were subjected tocontrolled cortical impact injury (CCII). PEDF mRNA brain levels were measured by RT-PCR. The lesion volume, cell proliferation, cell death and microglia activation were assessed in the brains of lesioned animals with intraventricular alzet infusion of PEDF or aCSF, and intraperitoneal injections of BrdU. Results: We detected a significant increase of PEDF mRNA levels after TBI. PEDF intraventricular infusions howed no significant effect on the contusion volume, whereas the number of dead cells, activated microglia, BrdU-positive cells around the lesion were significantly decreased. In contrast, PEDF application increased cell proliferation in the ipsilateral subventricular zone. No effect was found on cell proliferation in the dentate gyrus. Conclusion: The present work indicates that PEDF acts as a multifunctional agent after TBI influencing cell death, inflammation and cell proliferation.
UR - http://www.scopus.com/inward/record.url?scp=84920774477&partnerID=8YFLogxK
U2 - 10.3233/RNN-140417
DO - 10.3233/RNN-140417
M3 - Journal articles
C2 - 25420903
AN - SCOPUS:84920774477
SN - 0922-6028
VL - 33
SP - 81
EP - 93
JO - Restorative Neurology and Neuroscience
JF - Restorative Neurology and Neuroscience
IS - 1
ER -