Effects of endotoxin and catecholamines on hepatic mitochondrial respiration

Francesca Porta; Hendrik Bracht; Christian Weikert; Mario Beck; Jukka Takala; Sebastian Brandt; Luzius B. Hiltebrand; Stephan M. Jakob

doi:10.1007/s10753-009-9138-y

Effects of endotoxin and catecholamines on hepatic mitochondrial respiration

Francesca Porta, Hendrik Bracht, Christian Weikert, Mario Beck, Jukka Takala, Sebastian Brandt, Luzius B. Hiltebrand, Stephan M. Jakob

Department of Anesthesiology and Intensive Care Medicine

University of Bern

14 Citations (Scopus)

Abstract

Catecholamines are frequently used in sepsis, but their interaction with mitochondrial function is controversial. We incubated isolated native and endotoxin-exposed swine liver mitochondria with either dopamine, dobutamine, noradrenaline or placebo for 1 h. Mitochondrial State 3 and 4 respiration and their ratio (RCR) were determined for respiratory chain complexes I, II and IV. All catecholamines impaired glutamate-dependent RCR (p = 0.046), predominantly in native mitochondria. Endotoxin incubation alone induced a decrease in glutamate-dependent RCR compared to control samples (p = 0.002). We conclude that catecholamines and endotoxin impair the efficiency of mitochondrial complex I respiration in vitro.

Original language	English
Journal	Inflammation
Volume	32
Issue number	5
Pages (from-to)	315-321
Number of pages	7
ISSN	0360-3997
DOIs	https://doi.org/10.1007/s10753-009-9138-y
Publication status	Published - 10.2009

Funding

Supported by grant 3200BO/102268 from the Swiss National Fund.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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title = "Effects of endotoxin and catecholamines on hepatic mitochondrial respiration",

abstract = "Catecholamines are frequently used in sepsis, but their interaction with mitochondrial function is controversial. We incubated isolated native and endotoxin-exposed swine liver mitochondria with either dopamine, dobutamine, noradrenaline or placebo for 1 h. Mitochondrial State 3 and 4 respiration and their ratio (RCR) were determined for respiratory chain complexes I, II and IV. All catecholamines impaired glutamate-dependent RCR (p = 0.046), predominantly in native mitochondria. Endotoxin incubation alone induced a decrease in glutamate-dependent RCR compared to control samples (p = 0.002). We conclude that catecholamines and endotoxin impair the efficiency of mitochondrial complex I respiration in vitro.",

author = "Francesca Porta and Hendrik Bracht and Christian Weikert and Mario Beck and Jukka Takala and Sebastian Brandt and Hiltebrand, \{Luzius B.\} and Jakob, \{Stephan M.\}",

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T1 - Effects of endotoxin and catecholamines on hepatic mitochondrial respiration

AU - Porta, Francesca

AU - Bracht, Hendrik

AU - Weikert, Christian

AU - Beck, Mario

AU - Takala, Jukka

AU - Brandt, Sebastian

AU - Hiltebrand, Luzius B.

AU - Jakob, Stephan M.

PY - 2009/10

Y1 - 2009/10

N2 - Catecholamines are frequently used in sepsis, but their interaction with mitochondrial function is controversial. We incubated isolated native and endotoxin-exposed swine liver mitochondria with either dopamine, dobutamine, noradrenaline or placebo for 1 h. Mitochondrial State 3 and 4 respiration and their ratio (RCR) were determined for respiratory chain complexes I, II and IV. All catecholamines impaired glutamate-dependent RCR (p = 0.046), predominantly in native mitochondria. Endotoxin incubation alone induced a decrease in glutamate-dependent RCR compared to control samples (p = 0.002). We conclude that catecholamines and endotoxin impair the efficiency of mitochondrial complex I respiration in vitro.

AB - Catecholamines are frequently used in sepsis, but their interaction with mitochondrial function is controversial. We incubated isolated native and endotoxin-exposed swine liver mitochondria with either dopamine, dobutamine, noradrenaline or placebo for 1 h. Mitochondrial State 3 and 4 respiration and their ratio (RCR) were determined for respiratory chain complexes I, II and IV. All catecholamines impaired glutamate-dependent RCR (p = 0.046), predominantly in native mitochondria. Endotoxin incubation alone induced a decrease in glutamate-dependent RCR compared to control samples (p = 0.002). We conclude that catecholamines and endotoxin impair the efficiency of mitochondrial complex I respiration in vitro.

UR - https://www.scopus.com/pages/publications/70349669441

U2 - 10.1007/s10753-009-9138-y

DO - 10.1007/s10753-009-9138-y

M3 - Journal articles

C2 - 19603262

AN - SCOPUS:70349669441

SN - 0360-3997

VL - 32

SP - 315

EP - 321

JO - Inflammation

JF - Inflammation

IS - 5

ER -

Effects of endotoxin and catecholamines on hepatic mitochondrial respiration

Abstract

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