Effects of endotoxin and catecholamines on hepatic mitochondrial respiration

Francesca Porta, Hendrik Bracht, Christian Weikert, Mario Beck, Jukka Takala, Sebastian Brandt, Luzius B. Hiltebrand, Stephan M. Jakob

12 Citations (Scopus)


Catecholamines are frequently used in sepsis, but their interaction with mitochondrial function is controversial. We incubated isolated native and endotoxin-exposed swine liver mitochondria with either dopamine, dobutamine, noradrenaline or placebo for 1 h. Mitochondrial State 3 and 4 respiration and their ratio (RCR) were determined for respiratory chain complexes I, II and IV. All catecholamines impaired glutamate-dependent RCR (p = 0.046), predominantly in native mitochondria. Endotoxin incubation alone induced a decrease in glutamate-dependent RCR compared to control samples (p = 0.002). We conclude that catecholamines and endotoxin impair the efficiency of mitochondrial complex I respiration in vitro.

Original languageEnglish
Issue number5
Pages (from-to)315-321
Number of pages7
Publication statusPublished - 10.2009


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