Effects of different scaffolds on rat adipose tissue derived stroma cells

Yahya Açil; Xiacong Zhang; Tobias Nitsche; Björn Möller; Volker Gassling; Jörg Wiltfang; Matthias Gierloff

doi:10.1016/j.jcms.2013.11.020

Effects of different scaffolds on rat adipose tissue derived stroma cells

Yahya Açil, Xiacong Zhang, Tobias Nitsche, Björn Möller, Volker Gassling, Jörg Wiltfang, Matthias Gierloff^*

^*Corresponding author for this work

Clinic of Plastic Surgery

University of Kiel

17 Citations (Scopus)

Abstract

Background Adipose tissue derived stroma cells (ASC's) offer for many advantages for tissue engineering strategies over mesenchymal stroma cells from other sources and ideal carrier materials have to be identified for them. The aim of this study was to demonstrate and to compare the effects of three clinically established biomaterials on proliferation and metabolic activity of rat ASC's in vitro. Materials and methods Rat adipose tissue derived stroma cells (ASC's) were isolated and differentiated into distinct lineages proved by lineage specific staining and gene expression analysis (RT-PCR). The biomaterials Bio-Gide^®, Tutodent^® Membrane and Belotero^® Soft were tested with rat ASC's for their biocompatibility using scanning electron microscopy (SEM), cell vitality staining, cytotoxicity and proliferation tests (LDH, MTT, BrdU, WST-1). Results The collagen membrane Bio-Gide^® resulted in a significantly higher viability and proliferation (WST-1, BrdU) compared to Tutodent ^® Membrane. No significant difference was determined in the LDH and MTT test. The hyaluronic acid gel Belotero^® Soft showed no cytotoxicity (LDH, FDA/PI) and had no negative effects on metabolic activity (WST-1, MTT) or cell proliferation (BrdU) of ASC's. Conclusion Our results indicate Bio-Gide^® and Belotero^® Soft as preferable carrier materials for ASC's. For the further establishment of ASC's-based treatment strategies, in vivo investigations on the tissue regeneration potential of these cell-biomaterial scaffolds should follow.

Original language	English
Journal	Journal of Cranio-Maxillofacial Surgery
Volume	42
Issue number	6
Pages (from-to)	825-834
Number of pages	10
ISSN	1010-5182
DOIs	https://doi.org/10.1016/j.jcms.2013.11.020
Publication status	Published - 01.01.2014

Funding

The authors thank the Faculty of Medicine of the Christians-Albrechts University of Kiel for their financial support of this study. They also thank Mrs. Refrath and Mrs. Neßenius for their kind support and technical assistance in the laboratory.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jcms.2013.11.020

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title = "Effects of different scaffolds on rat adipose tissue derived stroma cells",

abstract = "Background Adipose tissue derived stroma cells (ASC's) offer for many advantages for tissue engineering strategies over mesenchymal stroma cells from other sources and ideal carrier materials have to be identified for them. The aim of this study was to demonstrate and to compare the effects of three clinically established biomaterials on proliferation and metabolic activity of rat ASC's in vitro. Materials and methods Rat adipose tissue derived stroma cells (ASC's) were isolated and differentiated into distinct lineages proved by lineage specific staining and gene expression analysis (RT-PCR). The biomaterials Bio-Gide{\textregistered}, Tutodent{\textregistered} Membrane and Belotero{\textregistered} Soft were tested with rat ASC's for their biocompatibility using scanning electron microscopy (SEM), cell vitality staining, cytotoxicity and proliferation tests (LDH, MTT, BrdU, WST-1). Results The collagen membrane Bio-Gide{\textregistered} resulted in a significantly higher viability and proliferation (WST-1, BrdU) compared to Tutodent {\textregistered} Membrane. No significant difference was determined in the LDH and MTT test. The hyaluronic acid gel Belotero{\textregistered} Soft showed no cytotoxicity (LDH, FDA/PI) and had no negative effects on metabolic activity (WST-1, MTT) or cell proliferation (BrdU) of ASC's. Conclusion Our results indicate Bio-Gide{\textregistered} and Belotero{\textregistered} Soft as preferable carrier materials for ASC's. For the further establishment of ASC's-based treatment strategies, in vivo investigations on the tissue regeneration potential of these cell-biomaterial scaffolds should follow.",

author = "Yahya A{\c c}il and Xiacong Zhang and Tobias Nitsche and Bj{\"o}rn M{\"o}ller and Volker Gassling and J{\"o}rg Wiltfang and Matthias Gierloff",

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doi = "10.1016/j.jcms.2013.11.020",

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T1 - Effects of different scaffolds on rat adipose tissue derived stroma cells

AU - Açil, Yahya

AU - Zhang, Xiacong

AU - Nitsche, Tobias

AU - Möller, Björn

AU - Gassling, Volker

AU - Wiltfang, Jörg

AU - Gierloff, Matthias

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Background Adipose tissue derived stroma cells (ASC's) offer for many advantages for tissue engineering strategies over mesenchymal stroma cells from other sources and ideal carrier materials have to be identified for them. The aim of this study was to demonstrate and to compare the effects of three clinically established biomaterials on proliferation and metabolic activity of rat ASC's in vitro. Materials and methods Rat adipose tissue derived stroma cells (ASC's) were isolated and differentiated into distinct lineages proved by lineage specific staining and gene expression analysis (RT-PCR). The biomaterials Bio-Gide®, Tutodent® Membrane and Belotero® Soft were tested with rat ASC's for their biocompatibility using scanning electron microscopy (SEM), cell vitality staining, cytotoxicity and proliferation tests (LDH, MTT, BrdU, WST-1). Results The collagen membrane Bio-Gide® resulted in a significantly higher viability and proliferation (WST-1, BrdU) compared to Tutodent ® Membrane. No significant difference was determined in the LDH and MTT test. The hyaluronic acid gel Belotero® Soft showed no cytotoxicity (LDH, FDA/PI) and had no negative effects on metabolic activity (WST-1, MTT) or cell proliferation (BrdU) of ASC's. Conclusion Our results indicate Bio-Gide® and Belotero® Soft as preferable carrier materials for ASC's. For the further establishment of ASC's-based treatment strategies, in vivo investigations on the tissue regeneration potential of these cell-biomaterial scaffolds should follow.

AB - Background Adipose tissue derived stroma cells (ASC's) offer for many advantages for tissue engineering strategies over mesenchymal stroma cells from other sources and ideal carrier materials have to be identified for them. The aim of this study was to demonstrate and to compare the effects of three clinically established biomaterials on proliferation and metabolic activity of rat ASC's in vitro. Materials and methods Rat adipose tissue derived stroma cells (ASC's) were isolated and differentiated into distinct lineages proved by lineage specific staining and gene expression analysis (RT-PCR). The biomaterials Bio-Gide®, Tutodent® Membrane and Belotero® Soft were tested with rat ASC's for their biocompatibility using scanning electron microscopy (SEM), cell vitality staining, cytotoxicity and proliferation tests (LDH, MTT, BrdU, WST-1). Results The collagen membrane Bio-Gide® resulted in a significantly higher viability and proliferation (WST-1, BrdU) compared to Tutodent ® Membrane. No significant difference was determined in the LDH and MTT test. The hyaluronic acid gel Belotero® Soft showed no cytotoxicity (LDH, FDA/PI) and had no negative effects on metabolic activity (WST-1, MTT) or cell proliferation (BrdU) of ASC's. Conclusion Our results indicate Bio-Gide® and Belotero® Soft as preferable carrier materials for ASC's. For the further establishment of ASC's-based treatment strategies, in vivo investigations on the tissue regeneration potential of these cell-biomaterial scaffolds should follow.

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Effects of different scaffolds on rat adipose tissue derived stroma cells

Abstract

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