TY - JOUR
T1 - Effects of dietary omega-3 and -6 supplementations on phospholipid fatty acid composition in mice uterus during window of pre-implantation
AU - Fattahi, Amir
AU - Darabi, Masoud
AU - Farzadi, Laya
AU - Salmassi, Ali
AU - Latifi, Zeinab
AU - Mehdizadeh, Amir
AU - Shaaker, Maghsood
AU - Ghasemnejad, Tohid
AU - Roshangar, Leila
AU - Nouri, Mohammad
N1 - Funding Information:
The study was financially supported by the Women's Reproductive Health Research Center , Tabriz University of Medical Sciences (grant number of 949/14.07.15 ) and the paper was extracted from PhD dissertation of Amir Fattahi.
Funding Information:
The study was financially supported by the Women's Reproductive Health Research Center, Tabriz University of Medical Sciences (grant number of 949/14.07.15) and the paper was extracted from PhD dissertation of Amir Fattahi.
Publisher Copyright:
© 2017 Elsevier Inc.
PY - 2018/3/1
Y1 - 2018/3/1
N2 - Since fatty acid composition of uterus phospholipids is likely to influence embryo implantation, this study was conducted to investigate the effects of dietary omega-3 and -6 fatty acids on implantation rate as well as uterine phospholipid fatty acids composition during mice pre-implantation period. Sixty female mice were randomly distributed into:1) control (standard pellet), 2) omega-3 (standard pellet + 10% w/w of omega-3 fatty acids) and 3) omega-6 (standard pellet + 10% w/w of omega-6 fatty acids). Uterine phospholipid fatty acid composition during the pre-implantation window (days 1–5 of pregnancy) was analyzed using gas-chromatography. The implantation rate on the fifth day of pregnancy was also determined. Our results showed that on days 1, 2 and 3 of pregnancy, the levels of arachidonic acid (ARA) as well as total omega-6 fatty acids were significantly higher and the levels of linolenic acid and total omega-3 fatty acids were statistically lower in the omega-6 group compared to the omega-3 group (p < 0.05). On the fourth day of pregnancy, only the ARA, total omega-6 fatty acids, and poly-unsaturated fatty acids levels were significantly different between the two dietary supplemented groups (p < 0.05). There were positive correlations between the levels of omega-6 fatty acids, especially ARA, with the implantation rate. The present study showed that diets rich in omega-3 and -6 fatty acids could differently modify uterine phospholipid fatty acid composition and uterine levels of phospholipid ARA, and that the total omega-6 fatty acids had a positive association with the implantation rate.
AB - Since fatty acid composition of uterus phospholipids is likely to influence embryo implantation, this study was conducted to investigate the effects of dietary omega-3 and -6 fatty acids on implantation rate as well as uterine phospholipid fatty acids composition during mice pre-implantation period. Sixty female mice were randomly distributed into:1) control (standard pellet), 2) omega-3 (standard pellet + 10% w/w of omega-3 fatty acids) and 3) omega-6 (standard pellet + 10% w/w of omega-6 fatty acids). Uterine phospholipid fatty acid composition during the pre-implantation window (days 1–5 of pregnancy) was analyzed using gas-chromatography. The implantation rate on the fifth day of pregnancy was also determined. Our results showed that on days 1, 2 and 3 of pregnancy, the levels of arachidonic acid (ARA) as well as total omega-6 fatty acids were significantly higher and the levels of linolenic acid and total omega-3 fatty acids were statistically lower in the omega-6 group compared to the omega-3 group (p < 0.05). On the fourth day of pregnancy, only the ARA, total omega-6 fatty acids, and poly-unsaturated fatty acids levels were significantly different between the two dietary supplemented groups (p < 0.05). There were positive correlations between the levels of omega-6 fatty acids, especially ARA, with the implantation rate. The present study showed that diets rich in omega-3 and -6 fatty acids could differently modify uterine phospholipid fatty acid composition and uterine levels of phospholipid ARA, and that the total omega-6 fatty acids had a positive association with the implantation rate.
UR - http://www.scopus.com/inward/record.url?scp=85035792424&partnerID=8YFLogxK
U2 - 10.1016/j.theriogenology.2017.10.031
DO - 10.1016/j.theriogenology.2017.10.031
M3 - Journal articles
C2 - 29207295
AN - SCOPUS:85035792424
SN - 0093-691X
VL - 108
SP - 97
EP - 102
JO - Theriogenology
JF - Theriogenology
ER -
