Effect of the HIV-1 nucleocapsid protein on reverse transcriptase pause sites revealed by single molecule microscopy

A. Jouonang; F. Przybilla; J. Godet; K. K. Sharma; T. Restle; H. De Rocquigny; J. L. Darlix; C. Kenfack; P. Didier; Y. Mély

doi:10.1117/12.2003478

Effect of the HIV-1 nucleocapsid protein on reverse transcriptase pause sites revealed by single molecule microscopy

A. Jouonang, F. Przybilla, J. Godet, K. K. Sharma, T. Restle, H. De Rocquigny, J. L. Darlix, C. Kenfack, P. Didier, Y. Mély

Institute of Molecular Medicine

Université de Douala

Abstract

During reverse transcription, the HIV-1 RNA is converted by the reverse transcriptase (RT) into proviral DNA. RT is assisted by the HIV-1 nucleocapsid (NCp7) protein that notably increases the ability of RT to synthesize DNA through pause sites. Using single molecule FRET, we monitored the NCp7 effect on the binding of RT to nucleic acid sequences corresponding to two different pause sites. NCp7 was found to modify the distribution of RT orientations on the oligonucleotides and decrease the residence time of RT on one of the pause sites. These results give direct insight into the NCp7 molecular mechanism in reverse transcription.

Original language	English
Title of host publication	Proceedings Volume 8590, Single Molecule Spectroscopy and Superresolution Imaging VI
Volume	859009
Publisher	SPIE
Publication date	22.02.2013
ISBN (Print)	978-081949359-0
DOIs	https://doi.org/10.1117/12.2003478
Publication status	Published - 22.02.2013
Event	Single Molecule Spectroscopy and Superresolution Imaging VI - San Francisco, United States Duration: 02.02.2013 → 03.02.2013

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Jouonang, A., Przybilla, F., Godet, J., Sharma, K. K., Restle, T., De Rocquigny, H., Darlix, J. L., Kenfack, C., Didier, P., & Mély, Y. (2013). Effect of the HIV-1 nucleocapsid protein on reverse transcriptase pause sites revealed by single molecule microscopy. In Proceedings Volume 8590, Single Molecule Spectroscopy and Superresolution Imaging VI (Vol. 859009 ). (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 859009). SPIE. https://doi.org/10.1117/12.2003478

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title = "Effect of the HIV-1 nucleocapsid protein on reverse transcriptase pause sites revealed by single molecule microscopy",

abstract = "During reverse transcription, the HIV-1 RNA is converted by the reverse transcriptase (RT) into proviral DNA. RT is assisted by the HIV-1 nucleocapsid (NCp7) protein that notably increases the ability of RT to synthesize DNA through pause sites. Using single molecule FRET, we monitored the NCp7 effect on the binding of RT to nucleic acid sequences corresponding to two different pause sites. NCp7 was found to modify the distribution of RT orientations on the oligonucleotides and decrease the residence time of RT on one of the pause sites. These results give direct insight into the NCp7 molecular mechanism in reverse transcription.",

author = "A. Jouonang and F. Przybilla and J. Godet and Sharma, {K. K.} and T. Restle and {De Rocquigny}, H. and Darlix, {J. L.} and C. Kenfack and P. Didier and Y. M{\'e}ly",

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Jouonang, A, Przybilla, F, Godet, J, Sharma, KK, Restle, T, De Rocquigny, H, Darlix, JL, Kenfack, C, Didier, P & Mély, Y 2013, Effect of the HIV-1 nucleocapsid protein on reverse transcriptase pause sites revealed by single molecule microscopy. in Proceedings Volume 8590, Single Molecule Spectroscopy and Superresolution Imaging VI. vol. 859009 , Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 859009, SPIE, Single Molecule Spectroscopy and Superresolution Imaging VI , San Francisco, California, United States, 02.02.13. https://doi.org/10.1117/12.2003478

Effect of the HIV-1 nucleocapsid protein on reverse transcriptase pause sites revealed by single molecule microscopy. / Jouonang, A.; Przybilla, F.; Godet, J. et al.
Proceedings Volume 8590, Single Molecule Spectroscopy and Superresolution Imaging VI. Vol. 859009 SPIE, 2013. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 859009).

Research output: Chapters in Books/Reports/Conference Proceedings › Conference contribution › peer-review

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T1 - Effect of the HIV-1 nucleocapsid protein on reverse transcriptase pause sites revealed by single molecule microscopy

AU - Jouonang, A.

AU - Przybilla, F.

AU - Godet, J.

AU - Sharma, K. K.

AU - Restle, T.

AU - De Rocquigny, H.

AU - Darlix, J. L.

AU - Kenfack, C.

AU - Didier, P.

AU - Mély, Y.

PY - 2013/2/22

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N2 - During reverse transcription, the HIV-1 RNA is converted by the reverse transcriptase (RT) into proviral DNA. RT is assisted by the HIV-1 nucleocapsid (NCp7) protein that notably increases the ability of RT to synthesize DNA through pause sites. Using single molecule FRET, we monitored the NCp7 effect on the binding of RT to nucleic acid sequences corresponding to two different pause sites. NCp7 was found to modify the distribution of RT orientations on the oligonucleotides and decrease the residence time of RT on one of the pause sites. These results give direct insight into the NCp7 molecular mechanism in reverse transcription.

AB - During reverse transcription, the HIV-1 RNA is converted by the reverse transcriptase (RT) into proviral DNA. RT is assisted by the HIV-1 nucleocapsid (NCp7) protein that notably increases the ability of RT to synthesize DNA through pause sites. Using single molecule FRET, we monitored the NCp7 effect on the binding of RT to nucleic acid sequences corresponding to two different pause sites. NCp7 was found to modify the distribution of RT orientations on the oligonucleotides and decrease the residence time of RT on one of the pause sites. These results give direct insight into the NCp7 molecular mechanism in reverse transcription.

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SN - 978-081949359-0

VL - 859009

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Proceedings Volume 8590, Single Molecule Spectroscopy and Superresolution Imaging VI

PB - SPIE

T2 - Single Molecule Spectroscopy and Superresolution Imaging VI

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ER -

Jouonang A, Przybilla F, Godet J, Sharma KK, Restle T, De Rocquigny H et al. Effect of the HIV-1 nucleocapsid protein on reverse transcriptase pause sites revealed by single molecule microscopy. In Proceedings Volume 8590, Single Molecule Spectroscopy and Superresolution Imaging VI. Vol. 859009 . SPIE. 2013. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2003478

Effect of the HIV-1 nucleocapsid protein on reverse transcriptase pause sites revealed by single molecule microscopy

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