Effect of pesticides on cell survival in liver and brain rat tissues

Mariana Astiz; María J.T.de Alaniz; Carlos Alberto Marra

doi:10.1016/j.ecoenv.2009.05.001

Effect of pesticides on cell survival in liver and brain rat tissues

Mariana Astiz, María J.T.de Alaniz, Carlos Alberto Marra^*

^*Corresponding author for this work

Institute of Neurobiology

Universidad Nacional de La Plata

104 Citations (Scopus)

Abstract

Pesticides are the main environmental factor associated with the etiology of human neurodegenerative disorders such as Parkinson's disease. Our laboratory has previously demonstrated that the treatment of rats with low doses of dimethoate, zineb or glyphosate alone or in combination induces oxidative stress (OS) in liver and brain. The aim of the present work was to investigate if the pesticide-induced OS was able to affect brain and liver cell survival. The treatment of Wistar rats with the pesticides (i.p. 1/250 LD50, three times a week for 5 weeks) caused loss of mitochondrial transmembrane potential and cardiolipin content, especially in substantia nigra (SN), with a concomitant increase of fatty acid peroxidation. The activation of calpain apoptotic cascade (instead of the caspase-dependent pathway) would be responsible for the DNA fragmentation pattern observed. Thus, these results may contribute to understand the effect(s) of chronic and simultaneous exposure to pesticides on cell survival.

Original language	English
Journal	Ecotoxicology and Environmental Safety
Volume	72
Issue number	7
Pages (from-to)	2025-2032
Number of pages	8
ISSN	0147-6513
DOIs	https://doi.org/10.1016/j.ecoenv.2009.05.001
Publication status	Published - 10.2009

Funding

This study was supported by a grant from Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and Universidad Nacional de La Plata (UNLP). We would like to thank Miss Norma Cristalli for her excellent technical assistance.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being
SDG 8 Decent Work and Economic Growth
SDG 10 Reduced Inequalities

Research Areas and Centers

Academic Focus: Center for Brain, Behavior and Metabolism (CBBM)

Access to Document

10.1016/j.ecoenv.2009.05.001

Cite this

@article{fb764d7b122b4b888e1352a312c7d8ba,

title = "Effect of pesticides on cell survival in liver and brain rat tissues",

abstract = "Pesticides are the main environmental factor associated with the etiology of human neurodegenerative disorders such as Parkinson's disease. Our laboratory has previously demonstrated that the treatment of rats with low doses of dimethoate, zineb or glyphosate alone or in combination induces oxidative stress (OS) in liver and brain. The aim of the present work was to investigate if the pesticide-induced OS was able to affect brain and liver cell survival. The treatment of Wistar rats with the pesticides (i.p. 1/250 LD50, three times a week for 5 weeks) caused loss of mitochondrial transmembrane potential and cardiolipin content, especially in substantia nigra (SN), with a concomitant increase of fatty acid peroxidation. The activation of calpain apoptotic cascade (instead of the caspase-dependent pathway) would be responsible for the DNA fragmentation pattern observed. Thus, these results may contribute to understand the effect(s) of chronic and simultaneous exposure to pesticides on cell survival.",

author = "Mariana Astiz and Alaniz, \{Mar{\'i}a J.T.de\} and Marra, \{Carlos Alberto\}",

note = "Funding Information: This study was supported by a grant from Consejo Nacional de Investigaciones Cient{\'i}ficas y T{\'e}cnicas (CONICET) and Universidad Nacional de La Plata (UNLP). We would like to thank Miss Norma Cristalli for her excellent technical assistance. Copyright: Copyright 2011 Elsevier B.V., All rights reserved.",

year = "2009",

month = oct,

doi = "10.1016/j.ecoenv.2009.05.001",

language = "English",

volume = "72",

pages = "2025--2032",

journal = "Ecotoxicology and Environmental Safety",

issn = "0147-6513",

publisher = "Academic Press",

number = "7",

}

TY - JOUR

T1 - Effect of pesticides on cell survival in liver and brain rat tissues

AU - Astiz, Mariana

AU - Alaniz, María J.T.de

AU - Marra, Carlos Alberto

N1 - Funding Information: This study was supported by a grant from Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and Universidad Nacional de La Plata (UNLP). We would like to thank Miss Norma Cristalli for her excellent technical assistance. Copyright: Copyright 2011 Elsevier B.V., All rights reserved.

PY - 2009/10

Y1 - 2009/10

N2 - Pesticides are the main environmental factor associated with the etiology of human neurodegenerative disorders such as Parkinson's disease. Our laboratory has previously demonstrated that the treatment of rats with low doses of dimethoate, zineb or glyphosate alone or in combination induces oxidative stress (OS) in liver and brain. The aim of the present work was to investigate if the pesticide-induced OS was able to affect brain and liver cell survival. The treatment of Wistar rats with the pesticides (i.p. 1/250 LD50, three times a week for 5 weeks) caused loss of mitochondrial transmembrane potential and cardiolipin content, especially in substantia nigra (SN), with a concomitant increase of fatty acid peroxidation. The activation of calpain apoptotic cascade (instead of the caspase-dependent pathway) would be responsible for the DNA fragmentation pattern observed. Thus, these results may contribute to understand the effect(s) of chronic and simultaneous exposure to pesticides on cell survival.

AB - Pesticides are the main environmental factor associated with the etiology of human neurodegenerative disorders such as Parkinson's disease. Our laboratory has previously demonstrated that the treatment of rats with low doses of dimethoate, zineb or glyphosate alone or in combination induces oxidative stress (OS) in liver and brain. The aim of the present work was to investigate if the pesticide-induced OS was able to affect brain and liver cell survival. The treatment of Wistar rats with the pesticides (i.p. 1/250 LD50, three times a week for 5 weeks) caused loss of mitochondrial transmembrane potential and cardiolipin content, especially in substantia nigra (SN), with a concomitant increase of fatty acid peroxidation. The activation of calpain apoptotic cascade (instead of the caspase-dependent pathway) would be responsible for the DNA fragmentation pattern observed. Thus, these results may contribute to understand the effect(s) of chronic and simultaneous exposure to pesticides on cell survival.

UR - https://www.scopus.com/pages/publications/70249120657

U2 - 10.1016/j.ecoenv.2009.05.001

DO - 10.1016/j.ecoenv.2009.05.001

M3 - Journal articles

C2 - 19493570

AN - SCOPUS:70249120657

SN - 0147-6513

VL - 72

SP - 2025

EP - 2032

JO - Ecotoxicology and Environmental Safety

JF - Ecotoxicology and Environmental Safety

IS - 7

ER -

Effect of pesticides on cell survival in liver and brain rat tissues

Abstract

Funding

UN SDGs

Research Areas and Centers

Access to Document

Other files and links

Fingerprint

Cite this