Abstract
Objective(s): Oleate can be produced through de novo synthesis, which contributes to biological processes and signaling pathways. However, the role of this non-essential fatty acid in hepatic development remains unclear. The current study aimed to evaluate the influence of early oleate deficiency induced by the inhibitor of de novo oleate synthesis MF-438 on fetal rat liver development. Materials and Methods: Female Wistar rats with an average weight of 200±20 g were subjected to this study. After mating, pregnant rats were divided into three groups and gavaged with the vehicle, MF 438 or MF-438 plus oleate from day 3 of pregnancy for five days. Obtained fetuses were sacrificed and the liver tissues were retrieved. Hepatic morphological index, biochemical markers, and gene expression of hepatic development markers were analyzed using Hematoxylin-Eosine, spectrometry, and real-time PCR techniques, respectively. Results: Relatively, deficient morphological indices and hepatic maturation markers were observed in fetus livers of the inhibitor-treated group. In comparison to the other two groups, total hepatic protein and glycogen content were increased with treatment of MF-438 plus oleate. Hepatocyte nuclear factor 1α, alpha fetoprotein, albumin, and cytochrome P450 gene expression were also significantly increased in the group treated with both MF-438 and oleate. Conclusion: Our data indicate that oleate availability during early embryo development is linked with fetal rat liver development.
| Original language | English |
|---|---|
| Journal | Iranian Journal of Basic Medical Sciences |
| Volume | 22 |
| Issue number | 9 |
| Pages (from-to) | 1010-1015 |
| Number of pages | 6 |
| ISSN | 2008-3866 |
| DOIs | |
| Publication status | Published - 01.06.2019 |
Funding
Th?s resear?h was 퀀謀nan??ally supported by a grant (grant no. 273) from the Liver and Gastrointestinal Disease Research Center of Tabriz University of Medical Sciences, Tabriz, Iran. The results presented in this paper were part of a PhD dissertation for FM with the supervision of MD. The authors appreciate the Iranian Council for Development of Stem Cell Sciences and Technologies for support of this work.
