Early immunohistochemical and functional markers indicating radiation damage of the parotid gland.

S. G. Hakim; H. C. Jacobsen; D. Hermes; H. Kosmehl; I. Lauer; R. Nadrowitz; P. Sieg

doi:10.1007/s00784-003-0249-9

Early immunohistochemical and functional markers indicating radiation damage of the parotid gland.

S. G. Hakim^*, H. C. Jacobsen, D. Hermes, H. Kosmehl, I. Lauer, R. Nadrowitz, P. Sieg

^*Corresponding author for this work

13 Citations (Scopus)

Abstract

In order to evaluate the correlation between functional impairment and changes in the expression pattern of immunohistochemical antibodies in the early phase of radiation-induced dysfunction of salivary glands, eight rabbits were scintigraphically examined prior to and 24 h after irradiation with 15 Gy. The parotid glands were studied using HE-staining, Ki-67, alpha-smooth muscle actin (ASMA) and tenascin-C antibodies at every scintigraphic examination. The results demonstrated a significant alteration in the 99mTc-pertechnetate uptake in all irradiated glands. HE-staining showed no relevant impairment of salivary gland tissue in this early phase. Immunohistochemically, we observed a marked re-distribution of ASMA and tenascin-C as well as a reduction of the proliferating rate of acinar cells. This immunohistochemical change correlated with the functional impairment manifested scintigraphically. This study proves the possibility to assess disorders of salivary gland function with immunohistological antibodies as early as 24 h after irradiation and yields the prerequisites to prove the effects of radioprotective agents on salivary gland tissues.

Original language	English
Journal	Clinical Oral Investigations
Volume	8
Issue number	1
Pages (from-to)	30-35
Number of pages	6
ISSN	1432-6981
DOIs	https://doi.org/10.1007/s00784-003-0249-9
Publication status	Published - 01.01.2004

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title = "Early immunohistochemical and functional markers indicating radiation damage of the parotid gland.",

abstract = "In order to evaluate the correlation between functional impairment and changes in the expression pattern of immunohistochemical antibodies in the early phase of radiation-induced dysfunction of salivary glands, eight rabbits were scintigraphically examined prior to and 24 h after irradiation with 15 Gy. The parotid glands were studied using HE-staining, Ki-67, alpha-smooth muscle actin (ASMA) and tenascin-C antibodies at every scintigraphic examination. The results demonstrated a significant alteration in the 99mTc-pertechnetate uptake in all irradiated glands. HE-staining showed no relevant impairment of salivary gland tissue in this early phase. Immunohistochemically, we observed a marked re-distribution of ASMA and tenascin-C as well as a reduction of the proliferating rate of acinar cells. This immunohistochemical change correlated with the functional impairment manifested scintigraphically. This study proves the possibility to assess disorders of salivary gland function with immunohistological antibodies as early as 24 h after irradiation and yields the prerequisites to prove the effects of radioprotective agents on salivary gland tissues.",

author = "Hakim, {S. G.} and Jacobsen, {H. C.} and D. Hermes and H. Kosmehl and I. Lauer and R. Nadrowitz and P. Sieg",

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T1 - Early immunohistochemical and functional markers indicating radiation damage of the parotid gland.

AU - Hakim, S. G.

AU - Jacobsen, H. C.

AU - Hermes, D.

AU - Kosmehl, H.

AU - Lauer, I.

AU - Nadrowitz, R.

AU - Sieg, P.

PY - 2004/1/1

Y1 - 2004/1/1

N2 - In order to evaluate the correlation between functional impairment and changes in the expression pattern of immunohistochemical antibodies in the early phase of radiation-induced dysfunction of salivary glands, eight rabbits were scintigraphically examined prior to and 24 h after irradiation with 15 Gy. The parotid glands were studied using HE-staining, Ki-67, alpha-smooth muscle actin (ASMA) and tenascin-C antibodies at every scintigraphic examination. The results demonstrated a significant alteration in the 99mTc-pertechnetate uptake in all irradiated glands. HE-staining showed no relevant impairment of salivary gland tissue in this early phase. Immunohistochemically, we observed a marked re-distribution of ASMA and tenascin-C as well as a reduction of the proliferating rate of acinar cells. This immunohistochemical change correlated with the functional impairment manifested scintigraphically. This study proves the possibility to assess disorders of salivary gland function with immunohistological antibodies as early as 24 h after irradiation and yields the prerequisites to prove the effects of radioprotective agents on salivary gland tissues.

AB - In order to evaluate the correlation between functional impairment and changes in the expression pattern of immunohistochemical antibodies in the early phase of radiation-induced dysfunction of salivary glands, eight rabbits were scintigraphically examined prior to and 24 h after irradiation with 15 Gy. The parotid glands were studied using HE-staining, Ki-67, alpha-smooth muscle actin (ASMA) and tenascin-C antibodies at every scintigraphic examination. The results demonstrated a significant alteration in the 99mTc-pertechnetate uptake in all irradiated glands. HE-staining showed no relevant impairment of salivary gland tissue in this early phase. Immunohistochemically, we observed a marked re-distribution of ASMA and tenascin-C as well as a reduction of the proliferating rate of acinar cells. This immunohistochemical change correlated with the functional impairment manifested scintigraphically. This study proves the possibility to assess disorders of salivary gland function with immunohistological antibodies as early as 24 h after irradiation and yields the prerequisites to prove the effects of radioprotective agents on salivary gland tissues.

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SN - 1432-6981

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SP - 30

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JO - Clinical Oral Investigations

JF - Clinical Oral Investigations

IS - 1

ER -

Early immunohistochemical and functional markers indicating radiation damage of the parotid gland.

Abstract

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