Dynamics and detection of laser induced micro bubbles in the retinal pigment epithelium (RPE)

Andreas Fritz*, Lars Ptaszynski, Hardo Stoehr, Ralf Brinkmann

*Corresponding author for this work


Selective Retina Treatment (SRT) is a new method to treat eye diseases associated with disorders of the RPE. Selective RPE cell damage is achieved by applying a train of 1.7 u.s laser pulses at 527 nm. The treatment of retinal diseases as e.g. diabetic maculopathy (DMP), is currently investigated within clinical studies, however 200 ns pulse durations are under investigation. Transient micro bubbles in the retinal pigment epithelium (RPE) are expected to be the origin of cell damage due to irradiation with laser pulses shorter than 50 u.s. The bubbles emerge at the strongly absorbing RPE melanosomes. Cell membrane disruption caused by the transient associated volume increase is expected to be the origin of the angiographically observed RPE leakage. We investigate micro bubble formation and dynamics in porcine RPE using pulse durations of 150 ns. A laser interferometry system at 830 nm with the aim of an online dosimetry control for SRT was developed. Bubble formation was detected interferometrically and by fast flash photography. A correlation to cell damage observed with a vitality stain is found. A bubble detection algorithm is presented.

Original languageEnglish
Title of host publicationTherapeutic Laser Applications and Laser-Tissue Interactions III
EditorsAlfred Vogel
Number of pages11
PublisherOptical Society of America
Publication date13.07.2007
Article number66321C
ISBN (Print)978-081946771-3
Publication statusPublished - 13.07.2007
EventEuropean Conference on Biomedical Optics 2007 - Munich, Germany
Duration: 17.06.200717.06.2007
Conference number: 104705

Research Areas and Centers

  • Academic Focus: Biomedical Engineering


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