Dynamic contrast in scanning microscopic OCT

Michael Münter*, Malte vom Endt, Mario Pieper, Malte Casper, Martin Ahrens, Tabea Kohlfaerber, Ramtin Rahmanzadeh, Peter König, Gereon Hüttmann, Hinnerk Schulz-Hildebrandt

*Corresponding author for this work


While optical coherence tomography (OCT) provides a resolution down to 1 µm it has difficulties to visualize cellular structures due to a lack of scattering contrast. By evaluating signal fluctuations, a significant contrast enhancement was demonstrated using time-domain full-field OCT (FF-OCT), which makes cellular and subcellular structures visible. The putative cause of the dynamic OCT signal is ATP-dependent motion of cellular structures in a sub-micrometer range, which provides histology-like contrast. Here we demonstrate dynamic contrast with a scanning frequency-domain OCT (FD-OCT). Given the inherent sectional imaging geometry, scanning FD-OCT provides depth-resolved images across tissue layers, a perspective known from histopathology, much faster and more efficiently than FF-OCT. Both, shorter acquisition times and tomographic depth-sectioning reduce the sensitivity of dynamic contrast for bulk tissue motion artifacts and simplify their correction in post-processing. The implementation of dynamic contrast makes microscopic FD-OCT a promising tool for histological analysis of unstained tissues.
Original languageEnglish
JournalOptics Letters
Issue number17
Pages (from-to)4766-4769
Number of pages4
Publication statusPublished - 28.02.2020

Research Areas and Centers

  • Academic Focus: Biomedical Engineering


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