Distribution of virulence genes related to adhesins and toxins in shiga toxin-producing escherichia coli strains isolated from healthy cattle and diarrheal patients in Japan

Yuluo Wu, Atsushi Hinenoya, Takashi Taguchi, Akira Nagita, Kensuke Shima, Teizo Tsukamoto, Nonhiko Sugimoto, Masahiro Asakura, Shinji Yamasaki*

*Corresponding author for this work
24 Citations (Scopus)

Abstract

Shiga toxin-producing Escherichia coli (STEC) isolated from Japan were investigated for the distribution of virulence genes. A total of 232 STEC strains including 171 from cattle and 61 from human were examined for the occurrence of genes responsible for bacterial adhesions to intestine, e.g., eae (intimin, E. coli attaching and effacing), saa (STEC autoagglutinating adhesin), iha (irgA homologue adhesin), efa1 (E. coli factor for adherence), lpfAO113 (long polar fimbriae), and ehaA (EHEC autotransporter) by colony hybridization assay. Similarly, the presence of toxigenic cdt (cytolethal distending toxin), and subAB (subtilase cytotoxin) genes were also checked. Among cattle isolates, 170, 163, 161, 155, 112 and 84 were positive for lpfAO113 (99%), ehaA (95%), iha (94%), saa (91%), subAB (65%), and cdt-V (49%), respectively, while 2 were positive for eae (1.2%) and efa1 (1.2%) each. In case of human isolates, 60, 59, 58 and 58 were positive for ehaA (98%), iha (97%), efa1 (95%), and eae (95%), respectively, while 11, 2, 2, and 1 were positive for lpfAO113 (18%), saa (3.3%), cdt-V (3.3%), and subAB (1.6%), respectively. Therefore, in human STEC isolates efa1 and eae whereas in cattle isolates saa, lpfAO113, cdt-V and subAB were prevalent. These data indicate differential occurrence of some pathogenic genes in human and cattle originated STEC strains in Japan.

Original languageEnglish
JournalJournal of Veterinary Medical Science
Volume72
Issue number5
Pages (from-to)589-597
Number of pages9
ISSN0916-7250
DOIs
Publication statusPublished - 25.06.2010

Research Areas and Centers

  • Academic Focus: Center for Infection and Inflammation Research (ZIEL)

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