Dissimilarity of airway and lung tissue microbiota in smokers undergoing surgery for lung cancer

Lena Reinhold; Andreas Möllering; Sönke Wallis; Emanuel Palade; Kathrin Schäfer; Daniel Drömann; Jan Rupp; Simon Graspeuntner; Klaus Dalhoff

doi:10.3390/microorganisms8060794

Dissimilarity of airway and lung tissue microbiota in smokers undergoing surgery for lung cancer

Lena Reinhold^*, Andreas Möllering, Sönke Wallis, Emanuel Palade, Kathrin Schäfer, Daniel Drömann, Jan Rupp, Simon Graspeuntner, Klaus Dalhoff

^*Corresponding author for this work

Abstract

Human airways are continuously colonized by microaspiration of microbiota. Less is known about the presence, origin and composition of microbiota in the lung parenchyma. In a study of 13 patients undergoing surgery for peripheral lung cancer microbiota composition was comparatively evaluated in upper airway, lower airway and lung tissue samples using 16S rDNA analysis. Bacterial density decreased stepwise from upper to lower airways and tissue. On a taxonomic level upper and lower airway microbiota were similar whereas lung tissue showed marked dissimilarities compared to lower airways that may reflect different environmental conditions shaping local microbiota and host immunity.

Original language	English
Article number	794
Journal	Microorganisms
Volume	8
Issue number	6
DOIs	https://doi.org/10.3390/microorganisms8060794
Publication status	Published - 06.2020

Funding

German Centre of Lung Research, Airway Research Centre North. The authors would like to thank the staff of the endoscopy unit, the department of surgery and the department of pathology of the University Hospital Schleswig-Holstein at Luebeck for valuable support of this study.

Research Areas and Centers

Academic Focus: Center for Infection and Inflammation Research (ZIEL)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/microorganisms8060794

Cite this

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title = "Dissimilarity of airway and lung tissue microbiota in smokers undergoing surgery for lung cancer",

abstract = "Human airways are continuously colonized by microaspiration of microbiota. Less is known about the presence, origin and composition of microbiota in the lung parenchyma. In a study of 13 patients undergoing surgery for peripheral lung cancer microbiota composition was comparatively evaluated in upper airway, lower airway and lung tissue samples using 16S rDNA analysis. Bacterial density decreased stepwise from upper to lower airways and tissue. On a taxonomic level upper and lower airway microbiota were similar whereas lung tissue showed marked dissimilarities compared to lower airways that may reflect different environmental conditions shaping local microbiota and host immunity.",

author = "Lena Reinhold and Andreas M{\"o}llering and S{\"o}nke Wallis and Emanuel Palade and Kathrin Sch{\"a}fer and Daniel Dr{\"o}mann and Jan Rupp and Simon Graspeuntner and Klaus Dalhoff",

note = "Funding Information: German Centre of Lung Research, Airway Research Centre North. The authors would like to thank the staff of the endoscopy unit, the department of surgery and the department of pathology of the University Hospital Schleswig-Holstein at Luebeck for valuable support of this study. Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

year = "2020",

month = jun,

doi = "10.3390/microorganisms8060794",

language = "English",

volume = "8",

journal = "Microorganisms",

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TY - JOUR

T1 - Dissimilarity of airway and lung tissue microbiota in smokers undergoing surgery for lung cancer

AU - Reinhold, Lena

AU - Möllering, Andreas

AU - Wallis, Sönke

AU - Palade, Emanuel

AU - Schäfer, Kathrin

AU - Drömann, Daniel

AU - Rupp, Jan

AU - Graspeuntner, Simon

AU - Dalhoff, Klaus

N1 - Funding Information: German Centre of Lung Research, Airway Research Centre North. The authors would like to thank the staff of the endoscopy unit, the department of surgery and the department of pathology of the University Hospital Schleswig-Holstein at Luebeck for valuable support of this study. Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/6

Y1 - 2020/6

N2 - Human airways are continuously colonized by microaspiration of microbiota. Less is known about the presence, origin and composition of microbiota in the lung parenchyma. In a study of 13 patients undergoing surgery for peripheral lung cancer microbiota composition was comparatively evaluated in upper airway, lower airway and lung tissue samples using 16S rDNA analysis. Bacterial density decreased stepwise from upper to lower airways and tissue. On a taxonomic level upper and lower airway microbiota were similar whereas lung tissue showed marked dissimilarities compared to lower airways that may reflect different environmental conditions shaping local microbiota and host immunity.

AB - Human airways are continuously colonized by microaspiration of microbiota. Less is known about the presence, origin and composition of microbiota in the lung parenchyma. In a study of 13 patients undergoing surgery for peripheral lung cancer microbiota composition was comparatively evaluated in upper airway, lower airway and lung tissue samples using 16S rDNA analysis. Bacterial density decreased stepwise from upper to lower airways and tissue. On a taxonomic level upper and lower airway microbiota were similar whereas lung tissue showed marked dissimilarities compared to lower airways that may reflect different environmental conditions shaping local microbiota and host immunity.

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U2 - 10.3390/microorganisms8060794

DO - 10.3390/microorganisms8060794

M3 - Journal articles

AN - SCOPUS:85085567387

VL - 8

JO - Microorganisms

JF - Microorganisms

IS - 6

M1 - 794

ER -

Dissimilarity of airway and lung tissue microbiota in smokers undergoing surgery for lung cancer

Abstract

Funding

Research Areas and Centers

UN SDGs

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