TY - JOUR
T1 - Differential expression of claudins in human endometrium and endometriosis
AU - Gaetje, Regine
AU - Holtrich, Uwe
AU - Engels, Knut
AU - Kissler, Stefan
AU - Rody, Achim
AU - Karn, Thomas
AU - Kaufmann, Manfred
N1 - Funding Information:
We thank Samira Adel and Katherina Kourtis for expert technical assistance. This work was supported by grants from the Deutsche Krebshilfe, Bonn; the Margarete Bonifer-Stiftung, Bad Soden; the BANSS-Stiftung, Biedenkopf; and the Dr. Robert Pfleger-Stiftung, Bamberg.
PY - 2008
Y1 - 2008
N2 - Membrane proteins of the claudin superfamily are important components of cellular tight and adherens junctions. Although their exact function remains unclear, these proteins may play a role in tissue remodeling, a process which is associated with several diseases including endometriosis. In the present work we analyzed the expression of 13 members of the claudin family in the endometrium and peritoneum by microarray analysis. Real-time polymerase chain reaction and immunohistochemistry in human endometrium and peritoneal endometriotic lesions were performed for validation of the expression of claudin-1, -3, -4, -5 and -7. Diminished expression of claudin-3, -4 and -7 in ectopic endometrium was frequently observed as indicated by all three methods. In contrast to a higher expression of claudin-5 mRNA detected in bulk biopsies of ectopic endometrium, immunohistochemistry revealed no alteration of claudin-5 protein expression in glandular cells of endometriosis samples. The downregulation of various members of the claudin family may contribute to endometrial cell detachment and increase the number of cells invading pelvic organs.
AB - Membrane proteins of the claudin superfamily are important components of cellular tight and adherens junctions. Although their exact function remains unclear, these proteins may play a role in tissue remodeling, a process which is associated with several diseases including endometriosis. In the present work we analyzed the expression of 13 members of the claudin family in the endometrium and peritoneum by microarray analysis. Real-time polymerase chain reaction and immunohistochemistry in human endometrium and peritoneal endometriotic lesions were performed for validation of the expression of claudin-1, -3, -4, -5 and -7. Diminished expression of claudin-3, -4 and -7 in ectopic endometrium was frequently observed as indicated by all three methods. In contrast to a higher expression of claudin-5 mRNA detected in bulk biopsies of ectopic endometrium, immunohistochemistry revealed no alteration of claudin-5 protein expression in glandular cells of endometriosis samples. The downregulation of various members of the claudin family may contribute to endometrial cell detachment and increase the number of cells invading pelvic organs.
UR - http://www.scopus.com/inward/record.url?scp=53849093400&partnerID=8YFLogxK
U2 - 10.1080/09513590802242694
DO - 10.1080/09513590802242694
M3 - Journal articles
C2 - 18850381
AN - SCOPUS:53849093400
SN - 0951-3590
VL - 24
SP - 442
EP - 449
JO - Gynecological Endocrinology
JF - Gynecological Endocrinology
IS - 8
ER -
